Key features and details
- Rabbit polyclonal to Cytokeratin 7
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cytokeratin 7 antibody
See all Cytokeratin 7 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 7
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Monkey
Recombinant fragment, corresponding to a region within amino acids 44-319 of Human Cytokeratin 7 (Uniprot ID: P08729).
- A431, HeLa and MOLT4 whole cell lysates; HeLa cells; Human placenta tissue and A549 xenograft tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 51 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionBlocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Tissue specificityExpressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling Cytokeratin 7 with ab154334 at 1/500 dilution.
Anti-Cytokeratin 7 antibody (ab154334) at 1/10000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 51 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Cytokeratin 7 with ab154334 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemical analysis of Human placenta tissue (paraformaldehyde-fixed frozen sections), labeling Cytokeratin 7 with ab154334 at 1/200 dilution.
ab154334 has been referenced in 1 publication.
- Wang Y & Li H Identification of proteins associated with paclitaxel resistance of epithelial ovarian cancer using iTRAQ-based proteomics. Oncol Lett 15:9793-9801 (2018). PubMed: 29928353