Key features and details
- Rabbit polyclonal to Cytokeratin 7
- Suitable for: IHC-P, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cytokeratin 7 antibody
See all Cytokeratin 7 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 7
Tested applicationsSuitable for: IHC-P, IP, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant full length protein corresponding to Human Cytokeratin 7 aa 2-469.
SIHFSSPVFTSRSAAFSGRGAQVRLSSARPGGLGSSSLYGLGASRPRVAV RSAYGGPVGAGIREVTINQSLLAPLRLDADPSLQRVRQEESEQIKTLNNK FASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQ LEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINHRTAAENEFVVLKKDV DAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDN SRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDL RNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDAR AKQEELEAALQRGKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEES RLAGDGVGAVNISVMNSTGGSSSGGGIGLTLGGTMGSNALSFSSSAGPGL LKAYSIRTASASRRSARD
Database link: P08729
- WB: HeLa, HepG2 and A549 whole cell lysates. IP: HepG2 whole cell lysate. IHC-P: Human liver and cervical cancer tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notes>95% pure
Our Abpromise guarantee covers the use of ab223051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|IP||1/200 - 1/2000.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).|
FunctionBlocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Tissue specificityExpressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
Paraffin embedded sections of human liver tissue were stained for Cytokeratin 7 with ab223051 at 1/100 dilution in immunohistochemical analysis.
Paraffin embedded sections of human cervical cancer tissue were stained for Cytokeratin 7 with ab223051 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Cytokeratin 7 (green) using ab223051 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
All lanes : Anti-Cytokeratin 7 antibody (ab223051) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat Anti_rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Cytokeratin 7 was immunoprecipitated from 0.5 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab223051 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab223051 at 1/500 dilution. HRP-conjugated Protein G was used as the secondary at 1/200 dilution.
Lane 1: Control IP with Rabbit monoclonal IgG
Lane 2: ab223051 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 μg (Input).
ab223051 has not yet been referenced specifically in any publications.