Recombinant
RabMAb

Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

Overview

  • Product name
    Anti-Cytokeratin 7 antibody [EP1620Y]
    See all Cytokeratin 7 primary antibodies
  • Description
    Rabbit monoclonal [EP1620Y] to Cytokeratin 7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Cytokeratin 7 aa 450-550 (C terminal).

  • Positive control
    • HeLa cell lysate; HeLa cells; T-47D; SK-BR-3; Human placenta.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EP1620Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP 1/20.

For unpurified use at 1/50. 

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
    • Tissue specificity
      Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Post-translational
      modifications
      Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CK 7 antibody
      • CK-7 antibody
      • CK7 antibody
      • Cytokeratin 7 antibody
      • Cytokeratin-7 antibody
      • D15Wsu77e antibody
      • K2C7 antibody
      • K2C7_HUMAN antibody
      • K7 antibody
      • Keratin 7 antibody
      • Keratin 7, type II antibody
      • Keratin type II cytoskeletal 7 antibody
      • Keratin, 55K type II cytoskeletal antibody
      • Keratin, simple epithelial antibody
      • Keratin, simple epithelial type I, K7 antibody
      • Keratin, type II cytoskeletal 7 antibody
      • Keratin-7 antibody
      • Krt2-7 antibody
      • KRT7 antibody
      • MGC11625 antibody
      • MGC129731 antibody
      • MGC3625 antibody
      • Sarcolectin antibody
      • SCL antibody
      • Type II mesothelial keratin K7 antibody
      • Type-II keratin Kb7 antibody
      see all

    Images

    • ab68460 (purified) at 1:20 dilution (0.6µg) immunoprecipitating Cytokeratin 7 in T-47D whole cell lysate.
      Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10μg
      Lane 2 (+): ab68460 & T-47D whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68460 in T-47D whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg/ml
      Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates at 20 µg/ml
      Lane 3 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg/ml
      Lane 4 : Human placenta lysates at 20 mg/ml

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 51 kDa



      Blocking and diluting buffer : 5% NFDM/TBST

      ~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform.[PMID:16265353]

    • Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (unpurified) + HeLa cell lysate at 10 µg

      Secondary
      HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 51 kDa
      Observed band size: 51 kDa

    References

    ab68460 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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