Product nameAnti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free
See all Cytokeratin 7 primary antibodies
DescriptionRabbit monoclonal [EPR1619Y] to Cytokeratin 7 - BSA and Azide free
Tested applicationsSuitable for: IP, IHC-P, WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cytokeratin 7 aa 1-100 (N terminal). The exact sequence is proprietary.
- IHC-P: Human bladder carcinoma tissue.
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
- Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 488) (ab185048)
- Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 647) (ab192077)
- Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (HRP) (ab192079)
- Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 594) (ab203427)
- Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 555) (ab203434)
- Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 568) (ab203435)
- Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Phycoerythrin) (ab210628)
- Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)
Our Abpromise guarantee covers the use of ab181831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|Flow Cyt||Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
FunctionBlocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Tissue specificityExpressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Cytokeratin 7 with purified ab68459 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68459).
ab181831 has not yet been referenced specifically in any publications.