RecombinantRabMAb

Recombinant Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 594) (ab203427)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1619Y] to Cytokeratin 7 - Cytoskeleton Marker (Alexa Fluor® 594)
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Overview

  • Product name

    Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 594)
    See all Cytokeratin 7 primary antibodies

  • Description

    Rabbit monoclonal [EPR1619Y] to Cytokeratin 7 - Cytoskeleton Marker (Alexa Fluor® 594)

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Cytokeratin 7 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: T47D cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Dissociation constant (KD)

    KD = 2.10 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1619Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Target

  • Function

    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

  • Tissue specificity

    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • CK 7 antibody
      • CK-7 antibody
      • CK7 antibody
      • Cytokeratin 7 antibody
      • Cytokeratin-7 antibody
      • D15Wsu77e antibody
      • K2C7 antibody
      • K2C7_HUMAN antibody
      • K7 antibody
      • Keratin 7 antibody
      • Keratin 7, type II antibody
      • Keratin type II cytoskeletal 7 antibody
      • Keratin, 55K type II cytoskeletal antibody
      • Keratin, simple epithelial antibody
      • Keratin, simple epithelial type I, K7 antibody
      • Keratin, type II cytoskeletal 7 antibody
      • Keratin-7 antibody
      • Krt2-7 antibody
      • KRT7 antibody
      • MGC11625 antibody
      • MGC129731 antibody
      • MGC3625 antibody
      • Sarcolectin antibody
      • SCL antibody
      • Type II mesothelial keratin K7 antibody
      • Type-II keratin Kb7 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 594) (ab203427)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 594) (ab203427)

      ab203427 staining Cytokeratin 7 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203427 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in T47D cells fixed with 100% methanol (5 min).

    • Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 594) (ab203427)
      Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 594) (ab203427)

    References (0)

    Publishing research using ab203427? Please let us know so that we can cite the reference in this datasheet.

    ab203427 has not yet been referenced specifically in any publications.

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