Recombinant Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 488) (ab208273)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 488)
See all Cytokeratin 7 primary antibodies -
Description
Rabbit monoclonal [EPR17078] to Cytokeratin 7 (Alexa Fluor® 488) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Mouse Cytokeratin 7 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9DCV7 -
Positive control
- ICC/IF: A549 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17078 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)
- Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 647) (ab209599)
- Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 594) (ab209600)
- Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 555) (ab209601)
- Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 568) (ab209602)
- Anti-Cytokeratin 7 antibody [EPR17078] (HRP) (ab209945)
- Anti-Cytokeratin 7 antibody [EPR17078] (Phycoerythrin) (ab213193)
- Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free (ab220804)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab208273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/5000. This product gave a positive signal in A549 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
Target
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Function
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
Tissue specificity
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3855 Human
- Entrez Gene: 110310 Mouse
- Entrez Gene: 300242 Rat
- Omim: 148059 Human
- SwissProt: P08729 Human
- SwissProt: Q9DCV7 Mouse
- SwissProt: Q6IG12 Rat
- Unigene: 411501 Human
see all -
Alternative names
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 488) (ab208273)](https://www.abcam.com/ps/products/208/ab208273/Images/ab208273-256393-anti-cytokeratin-7-antibody-epr17078-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 488) (ab208273)ab208273 staining Cytokeratin 7 in A549 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208273 at 1/5000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in A549 cells fixed with 4% formaldehyde (10 min).
Datasheets and documents
References
ab208273 has not yet been referenced specifically in any publications.
