Overview

  • Product name

    Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker
    See all Cytokeratin 7 primary antibodies
  • Description

    Rabbit monoclonal [EPR17078] to Cytokeratin 7 - Cytoskeleton Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse Cytokeratin 7 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9DCV7

  • Positive control

    • WB: Rat bladder and kidney, mouse lung, kidney and liver tissue lysates and HeLa cell lysate. IHC-P: Human mammary gland, mouse liver and rat liver tissue. IHC-F: Mouse kidney tissue. ICC/IF: A549 cells. Flow Cyt: A549 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17078
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1500.
IHC-Fr 1/8000.
WB 1/1000. Detects a band of approximately 51, 45, 42 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function

    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
  • Tissue specificity

    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CK 7 antibody
    • CK-7 antibody
    • CK7 antibody
    • Cytokeratin 7 antibody
    • Cytokeratin-7 antibody
    • D15Wsu77e antibody
    • K2C7 antibody
    • K2C7_HUMAN antibody
    • K7 antibody
    • Keratin 7 antibody
    • Keratin 7, type II antibody
    • Keratin type II cytoskeletal 7 antibody
    • Keratin, 55K type II cytoskeletal antibody
    • Keratin, simple epithelial antibody
    • Keratin, simple epithelial type I, K7 antibody
    • Keratin, type II cytoskeletal 7 antibody
    • Keratin-7 antibody
    • Krt2-7 antibody
    • KRT7 antibody
    • MGC11625 antibody
    • MGC129731 antibody
    • MGC3625 antibody
    • Sarcolectin antibody
    • SCL antibody
    • Type II mesothelial keratin K7 antibody
    • Type-II keratin Kb7 antibody
    see all

Images

  • Hepatic inflammation and abnormal intrahepatic bile ducts in the N-IF mouse

    Immunofluorescence staining of bile ducts (CK-7, red), macrophages (F4/80, light blue) and nuclei (DAPI, blue) in livers of 8 week old N-IF and 24αβNOD control mice. Representative images from two independent experiments with a total of six mice are shown. Scale bars are 100 μm.

    Cytokeratin is detected with ab181598 at1/1500 dilution in formalin-fixed, paraffin-embedded mouse liver tissue. The secondary antibody is an Anti-Rabbit AlexaFluor®594 conjugate (red).

    (From Figure 1D of Fransen-Pettersson, N. et al)

  • Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling Cytokeratin 7 with ab181598 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Membrane and cytoplasmic staining on epithelial cells of breast tissues is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A549  (Human lung carcinoma) cells  labeling Cytokeratin 7 with ab181598 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic staining on A549 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab181598 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • All lanes : Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598) at 1/50000 dilution

    Lane 1 : Rat bladder tissue
    Lane 2 : Mouse lung tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 52, 42 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

     

    ~45 and 42kDa bands are supported by the literature and competitor’s products, which may be the isoforms.

  • Immunohistochemical analysis of formaldehyde-fixed, frozen section of mouse salivary gland tissue labeling Cytokeratin 7 with ab181598 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG AlexaFluor®660.

    See Abreview

  • Flow cytometry analysis of A549 (human lung carcinoma) cells labeling Cytokeratin 7 (red) with ab181598 at a 1/1500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598) at 1/1000 dilution + Mouse liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa



    ~45 and 42kDa bands are supported by the literature and competitor’s products, which may be the isoforms.

  • Immunohistochemical analysis of frozen Mouse kidney tissue labeling Cytokeratin 7 with ab181598 at 1/8000 dilution, followed by Donkey anti-rabbit Alexa Fluor 594 at 1/1000 dilution. Cytoplasmic staining on collecting tube is observed. This data is from our collaborator Dr. Hai Song’s lab (Life Sciences Institute Zhejiang University). Counter stained with DAPI.

    Negative control: Using PBS instead of primary antibody.

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Cytokeratin 7 with ab181598 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. On mouse liver, only bile duct epithelia are positive, no reaction in hepatocytes is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598) at 1/20000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg
    Lane 2 : Rat kidney tissue lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51, 45, 42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

     

    ~45 and 42kDa bands are supported by the literature and competitor’s products, which may be the isoforms.

  • Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Cytokeratin 7 with ab181598 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. On rat liver, only bile duct epithelia are positive, no reaction in hepatocytes is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598) at 1/5000 dilution + Mouse kidney lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51, 45, 42 kDa why is the actual band size different from the predicted?



    ~45 and 42kDa bands are supported by the literature and competitor’s products, which may be the isoforms.

References

This product has been referenced in:

  • Gao Q  et al. Heterotypic CAF-tumor spheroids promote early peritoneal metastatis of ovarian cancer. J Exp Med 216:688-703 (2019). Read more (PubMed: 30710055) »
  • Wang Y  et al. Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease. Int J Nanomedicine 14:963-976 (2019). Read more (PubMed: 30787610) »
See all 25 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Urothelium)
Antigen retrieval step
None
Permeabilization
Yes - Triton 0,3% - 5 minutesr
Specification
Urothelium
Blocking step
Normal Goat Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 21 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse salivary gland)
Permeabilization
Yes - 0.1% Triton
Specification
Mouse salivary gland
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
Fixative
Formaldehyde

Dr. Isabelle Miletich

Verified customer

Submitted Mar 14 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Mar 07 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Syrian Hamster Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 12 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 12 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 03 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 21 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 21 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (oviduct, epithelial cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
No
Specification
oviduct, epithelial cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 26 2015

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