Product nameAnti-Cytokeratin 7 antibody [EPR17078] (Phycoerythrin)
See all Cytokeratin 7 primary antibodies
DescriptionRabbit monoclonal [EPR17078] to Cytokeratin 7 (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Mouse Cytokeratin 7 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9DCV7
- Flow Cyt: A549 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)
- Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 488) (ab208273)
- Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 647) (ab209599)
- Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 594) (ab209600)
- Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 555) (ab209601)
- Anti-Cytokeratin 7 antibody [EPR17078] (Alexa Fluor® 568) (ab209602)
- Anti-Cytokeratin 7 antibody [EPR17078] (HRP) (ab209945)
- Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free (ab220804)
Our Abpromise guarantee covers the use of ab213193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
FunctionBlocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Tissue specificityExpressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
Overlay histogram showing A549 cells stained with ab213193 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213193, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab213193 has not yet been referenced specifically in any publications.