Recombinant
RabMAb

Recombinant Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17079] to Cytokeratin 7
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Cytokeratin 7 antibody [EPR17079]
    See all Cytokeratin 7 primary antibodies

  • Description

    Rabbit monoclonal [EPR17079] to Cytokeratin 7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse Cytokeratin 7 aa 1-250. The exact sequence is proprietary.
    Database link: Q9DCV7

  • Positive control

    • WB: Mouse lung, bladder and skin lysates; Rat bladder and lung lysates. IHC-P: Mouse lung and colon tissues. IP: Mouse skin whole cell extract .

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17079

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/4000. Detects a band of approximately 45, 51 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Target

  • Function

    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

  • Tissue specificity

    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • CK 7 antibody
      • CK-7 antibody
      • CK7 antibody
      • Cytokeratin 7 antibody
      • Cytokeratin-7 antibody
      • D15Wsu77e antibody
      • K2C7 antibody
      • K2C7_HUMAN antibody
      • K7 antibody
      • Keratin 7 antibody
      • Keratin 7, type II antibody
      • Keratin type II cytoskeletal 7 antibody
      • Keratin, 55K type II cytoskeletal antibody
      • Keratin, simple epithelial antibody
      • Keratin, simple epithelial type I, K7 antibody
      • Keratin, type II cytoskeletal 7 antibody
      • Keratin-7 antibody
      • Krt2-7 antibody
      • KRT7 antibody
      • MGC11625 antibody
      • MGC129731 antibody
      • MGC3625 antibody
      • Sarcolectin antibody
      • SCL antibody
      • Type II mesothelial keratin K7 antibody
      • Type-II keratin Kb7 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Mouse bladder lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 51 kDa
      Observed band size: 45,51 kDa
      why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      ~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.

      Blocking/dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution + Mouse skin lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 51 kDa
      Observed band size: 45,51 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      ~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/4000 dilution

      Lane 1 : Rat bladder lysate
      Lane 2 : Rat lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 51 kDa
      Observed band size: 45,51 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      ~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

      Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

      Cytokeratin 7 was immunoprecipitated from 1mg of Mouse skin whole cell extract with ab199718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199718 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: Mouse skin whole cell extract 10 µg (Input). Lane 2: ab199718 IP in Mouse skin whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199718 in Mouse skin whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    References

    Publishing research using ab199718? Please let us know so that we can cite the reference in this datasheet.

    ab199718 has been referenced in 1 publication.

    • Musutova M  et al. The Effect of Hypoxia and Metformin on Fatty Acid Uptake, Storage, and Oxidation in L6 Differentiated Myotubes. Front Endocrinol (Lausanne) 9:616 (2018). PubMed: 30386299

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 7 antibody [EPR17079]

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse blastocyst)
    Permeabilization
    No
    Specification
    Mouse blastocyst
    Blocking step
    1% BSA in 10% normal goat serum in 0.1%PBS-Tween as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    4% PFA 5 mins RT then 100% methanol for 2mins at RT
    Read More

    Ms. Lisa Campbell

    Verified customer

    Submitted Sep 11 2018

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