For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    cytokeratin-7-antibody-epr17079-ab199718.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
Share by email
RecombinantRabMAb

Recombinant Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
  • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
  • Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17079] to Cytokeratin 7
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-CXCR4 antibody [UMB2] (ab124824)
Protein
Product image
Recombinant Human Cytokeratin 7 protein (ab132933)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-Cytokeratin 7 antibody [EPR17079]
    See all Cytokeratin 7 primary antibodies
  • Description

    Rabbit monoclonal [EPR17079] to Cytokeratin 7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung, bladder and skin lysates; Rat bladder and lung lysates. IHC-P: Mouse lung and colon tissues. IP: Mouse skin whole cell extract .
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17079
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Alternative Versions

    • Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Cytokeratin 7 protein (ab132933)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab199718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/4000. Detects a band of approximately 45, 51 kDa (predicted molecular weight: 51 kDa).
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
Notes
WB
1/4000. Detects a band of approximately 45, 51 kDa (predicted molecular weight: 51 kDa).
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.

Target

  • Function

    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
  • Tissue specificity

    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P08729 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 110310 Mouse
    • Entrez Gene: 300242 Rat
    • SwissProt: Q9DCV7 Mouse
    • SwissProt: Q6IG12 Rat
    • Unigene: 289377 Mouse
    • Unigene: 7913 Rat
    • Alternative names

      • CK 7 antibody
      • CK-7 antibody
      • CK7 antibody
      • Cytokeratin 7 antibody
      • Cytokeratin-7 antibody
      • D15Wsu77e antibody
      • K2C7 antibody
      • K2C7_HUMAN antibody
      • K7 antibody
      • Keratin 7 antibody
      • Keratin 7, type II antibody
      • Keratin type II cytoskeletal 7 antibody
      • Keratin, 55K type II cytoskeletal antibody
      • Keratin, simple epithelial antibody
      • Keratin, simple epithelial type I, K7 antibody
      • Keratin, type II cytoskeletal 7 antibody
      • Keratin-7 antibody
      • Krt2-7 antibody
      • KRT7 antibody
      • MGC11625 antibody
      • MGC129731 antibody
      • MGC3625 antibody
      • Sarcolectin antibody
      • SCL antibody
      • Type II mesothelial keratin K7 antibody
      • Type-II keratin Kb7 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Mouse bladder lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 51 kDa
      Observed band size: 45,51 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      ~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.

      Blocking/dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution + Mouse skin lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 51 kDa
      Observed band size: 45,51 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      ~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/4000 dilution

      Lane 1 : Rat bladder lysate
      Lane 2 : Rat lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 51 kDa
      Observed band size: 45,51 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      ~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

      Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

      Cytokeratin 7 was immunoprecipitated from 1mg of Mouse skin whole cell extract with ab199718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199718 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: Mouse skin whole cell extract 10 µg (Input). Lane 2: ab199718 IP in Mouse skin whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199718 in Mouse skin whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
      Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab199718? Please let us know so that we can cite the reference in this datasheet.

    ab199718 has been referenced in 1 publication.

    • Musutova M  et al. The Effect of Hypoxia and Metformin on Fatty Acid Uptake, Storage, and Oxidation in L6 Differentiated Myotubes. Front Endocrinol (Lausanne) 9:616 (2018). PubMed: 30386299

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 7 antibody [EPR17079]

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse blastocyst)
    Permeabilization
    No
    Specification
    Mouse blastocyst
    Blocking step
    1% BSA in 10% normal goat serum in 0.1%PBS-Tween as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    4% PFA 5 mins RT then 100% methanol for 2mins at RT
    Read More

    Ms. Lisa Campbell

    Verified customer

    Submitted Sep 11 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.