Recombinant Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)](https://www.abcam.com/ps/products/199/ab199718/Images/ab199718-242234-anti-cytokeratin-7-antibody-epr17079-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17079] to Cytokeratin 7
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 7 antibody [EPR17079]
See all Cytokeratin 7 primary antibodies -
Description
Rabbit monoclonal [EPR17079] to Cytokeratin 7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung, bladder and skin lysates; Rat bladder and lung lysates. IHC-P: Mouse lung and colon tissues. IP: Mouse skin whole cell extract .
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17079 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/4000. Detects a band of approximately 45, 51 kDa (predicted molecular weight: 51 kDa).
|
|
| IHC-P |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IP |
1/30.
|
| Notes |
|---|
|
WB
1/4000. Detects a band of approximately 45, 51 kDa (predicted molecular weight: 51 kDa). |
|
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
Target
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Function
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
Tissue specificity
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 110310 Mouse
- Entrez Gene: 300242 Rat
- SwissProt: Q9DCV7 Mouse
- SwissProt: Q6IG12 Rat
- Unigene: 289377 Mouse
- Unigene: 7913 Rat
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Alternative names
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)](https://www.abcam.com/ps/products/199/ab199718/Images/ab199718-242237-anti-cytokeratin-7-antibody-epr17079-western-blot.jpg)
Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse bladder lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 45,51 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.
Blocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)](https://www.abcam.com/ps/products/199/ab199718/Images/ab199718-242236-anti-cytokeratin-7-antibody-epr17079-western-blot.jpg)
Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution + Mouse skin lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 45,51 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)](https://www.abcam.com/ps/products/199/ab199718/Images/ab199718-242235-anti-cytokeratin-7-antibody-epr17079-western-blot.jpg)
Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/4000 dilution
Lane 1 : Rat bladder lysate
Lane 2 : Rat lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 45,51 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)](https://www.abcam.com/ps/products/199/ab199718/Images/ab199718-242233-anti-cytokeratin-7-antibody-epr17079-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)](https://www.abcam.com/ps/products/199/ab199718/Images/ab199718-242232-anti-cytokeratin-7-antibody-epr17079-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)Cytokeratin 7 was immunoprecipitated from 1mg of Mouse skin whole cell extract with ab199718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199718 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Mouse skin whole cell extract 10 µg (Input). Lane 2: ab199718 IP in Mouse skin whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199718 in Mouse skin whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. -
![Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)](https://www.abcam.com/ps/products/199/ab199718/Images/ab199718-8-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab199718 has been referenced in 1 publication.
- Musutova M et al. The Effect of Hypoxia and Metformin on Fatty Acid Uptake, Storage, and Oxidation in L6 Differentiated Myotubes. Front Endocrinol (Lausanne) 9:616 (2018). PubMed: 30386299