Overview

  • Product name
    Anti-Cytokeratin 7 antibody [RCK105]
    See all Cytokeratin 7 primary antibodies
  • Description
    Mouse monoclonal [RCK105] to Cytokeratin 7
  • Host species
    Mouse
  • Specificity
    This antibody reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ICC, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Hamster, Cat, Dog, Human, Pig, Zebrafish
  • Immunogen

    Cytokeratins from the human bladder carcinoma cell line T24.

  • Positive control
    • mouse ovary tissue, breast tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    RCK105
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/100 - 1/500.
ICC 1/100 - 1/500.
WB 1/100 - 1/1000.
ICC/IF Use at an assay dependent concentration. PubMed: 19233130
IHC-P 1/1000.

Target

  • Function
    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
  • Tissue specificity
    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CK 7 antibody
    • CK-7 antibody
    • CK7 antibody
    • Cytokeratin 7 antibody
    • Cytokeratin-7 antibody
    • D15Wsu77e antibody
    • K2C7 antibody
    • K2C7_HUMAN antibody
    • K7 antibody
    • Keratin 7 antibody
    • Keratin 7, type II antibody
    • Keratin type II cytoskeletal 7 antibody
    • Keratin, 55K type II cytoskeletal antibody
    • Keratin, simple epithelial antibody
    • Keratin, simple epithelial type I, K7 antibody
    • Keratin, type II cytoskeletal 7 antibody
    • Keratin-7 antibody
    • Krt2-7 antibody
    • KRT7 antibody
    • MGC11625 antibody
    • MGC129731 antibody
    • MGC3625 antibody
    • Sarcolectin antibody
    • SCL antibody
    • Type II mesothelial keratin K7 antibody
    • Type-II keratin Kb7 antibody
    see all

Images

  • Immunohistochemistry on frozen sections of human liver, only bile duct epithelium positive; no reaction in hepatocytes
  • ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.

  • ab9021 staining breast epithelial cell line. The cells are fixated in Methanol. These pictures are part of the review submitted by Rene Villadsen.
  • Formalin fixed parrafin embedded mouse ovary tissue. Ab9021 used at 1:1000 incubated with overnight incubation with a biotinylated secondary and peroxidase/DAB detection. Review by Todd Gocken submitted 21 July 2004

  • ab9021 staining Cytokeratin 7 in mouse liver, heterogeneous primary cell culture by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 3%  Mouse Ig Blocking reagent (component of a commercially available mouse on mouse MOM kit) at 200C for 30 minutes. Samples were incubated with primary antibody (1/100: in dilution buffer from MOM kit) for 1 hour at 200C. An Alexa Fluor ® 568-conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/250 dilution.

    See Abreview

  • All lanes : Anti-Cytokeratin 7 antibody [RCK105] (ab9021) at 1/200 dilution

    All lanes : Whole tissue lysate prepared from human pancreatic tumor tissue.

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-mouse HRP at 1/10000 dilution

    Developed using the ECL technique.

    Exposure time: 2 minutes


    Blocked with 3% BSA for 2 hours.

    See Abreview

  • Immunohistochemistry on frozen sections of swine liver, only bile duct epithelium positive; no reaction in hepatocytes

References

This product has been referenced in:
  • Kallergi G  et al. Evaluation of PD-L1/PD-1 on circulating tumor cells in patients with advanced non-small cell lung cancer. Ther Adv Med Oncol 10:1758834017750121 (2018). ICC/IF . Read more (PubMed: 29383038) »
  • Zhang W  et al. ARHGEF11 affecting the placental insulin signaling pathway in fetal macrosomia of normal glucose tolerance pregnant women. Placenta 63:7-14 (2018). IHC-P . Read more (PubMed: 29486856) »
See all 40 Publications for this product

Customer reviews and Q&As

1-10 of 22 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Salivary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0
Permeabilization
No
Specification
Salivary gland
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 20 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (thyroid follicular tumor, follicular cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
thyroid follicular tumor, follicular cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (oviductal tissues)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
Permeabilization
No
Specification
oviductal tissues
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 16 2016

Answer

Thank you for contacting us.

You had previously been in contact with my colleague heather regarding staining observed in your negative control using these mouse monoclonal Cytokeratin antibodies on mouse tissue. To clarify, is your negative control from a tissue that is not expected to express cytokeratin or is it an isotype control or a no primary antibody control?

When using an anti-mouse secondary antibody on mouse tissue, background staining is often observed due to cross-reactivity with endogenous mouse IgGs in the sample. This can be checked by testing a no-primary antibody control slide.

If you are observing mouse on mouse background staining, this can be avoided by either using a mouse on mouse (MOM) staining kit, such as ab127055. The background can also be blocked by adding an incubation with a F(ab) fragment unconjugated anti-mouse secondary antibody, such as ab6668. The F(ab) fragment will coat any endogenous IgGs in your sample, and can be done just before the serum block at a 1:500 dilution for 1 hour at room temperature.

I hope this helps, if not, please let me know and I will be happy to help you further.

Read More

Answer

I apologize for the delay.

Vimentin is a mesenchymal cell marker, ab8978 is tested in Zebrafish. It might be suitable for your purpose.

ab9004, ab9021, ab9217 are anti Cytokeratin 4,7 and 18. Against epithelial cell marker the antibodies is tested in Zebrafish.

Could you more specific to type of blood cell marker you are interested in?

We do not have anti M-Cadherin and Myo-D antibodies that could be used for Zebrafish. This assumption is based on the protein sequence similarity between human and Zebrafish. We unfortunately do not have antibodies against these target that might cross react.

I have attached a Excel file of all Zebrafish specific antibodies with immunogen sequence. This file will help you selecting the antibodies based on cross reactivity due to immunogen sequence homology.

I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

Read More

Question
Answer

Thank you for contacting us.

Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


Abpromise guarantee:

We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab52870.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Human pancreatic tumor)
Loading amount
20 µg
Specification
Human pancreatic tumor
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3%

Abcam user community

Verified customer

Submitted Nov 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Non-reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Dec 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Liver)
Specification
Liver
Fixative
95% ETOH
Permeabilization
No
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Dec 04 2010

1-10 of 22 Abreviews or Q&A

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