Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
Key features and details
- Mouse monoclonal [RCK105] to Cytokeratin 7 - Cytoskeleton Marker
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker
See all Cytokeratin 7 primary antibodies -
Description
Mouse monoclonal [RCK105] to Cytokeratin 7 - Cytoskeleton Marker -
Host species
Mouse -
Specificity
This antibody reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma. -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Rat, Goat, Hamster, Cat, Dog, Zebrafish -
Immunogen
Full length native protein (purified) corresponding to Human Cytokeratin 7. BALB/c mouse immunized with cytokeratins from the human bladder carcinoma cell line T24.
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Positive control
- IHC-P: Human liver tissue, mouse ovary and bladder tissue IHC-Fr: Human liver, urinary bladder tissue, Pig liver and skin tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RCK105 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr | (2) |
1/100 - 1/500.
|
IHC-P | (5) |
1/1000.
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Notes |
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IHC-Fr
1/100 - 1/500. |
IHC-P
1/1000. |
Target
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Function
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
Tissue specificity
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3855 Human
- Entrez Gene: 300242 Rat
- Omim: 148059 Human
- SwissProt: P08729 Human
- SwissProt: Q6IG12 Rat
- Unigene: 411501 Human
- Unigene: 670221 Human
- Unigene: 7913 Rat
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Alternative names
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
Images
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Immunohistochemistry analysis of formalin fixed, paraffin embedded sections of human liver tissue labeling Cytokeratin 7 with ab9021 at 1/100; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.
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ab9021 staining normal pig skin tissue section (frozen), staining epithelial cells using ab9021 at 1/500.
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Immunofluorescent staining of β-galactosideasae and cytokeratin expression of urinary bladder after intravesical treatment with Adeno-Cre
Formalin-fixed, paraffin-embedded mouse bladder tissue stained for β-galactosidase (green, A) or Cytokeratin 7 (red, B). Panel C shows the colocalization of β-galactosidase and Cytokeratin 7. The nuclear counterstain is DAPI (blue).
Cytokeratin 7 was detected using ab9021.
(After Figure 3 of Yang et al)
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ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.
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Immunohistochemistry analysis of frozen sections of human liver labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.
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Immunohistochemistry analysis of frozen sections of human urinary bladder labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells and no reactivity in connective tissue.
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Immunohistochemistry on frozen sections of human liver, only bile duct epithelium positive; no reaction in hepatocytes
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Immunohistochemistry on frozen sections of swine liver, only bile duct epithelium positive; no reaction in hepatocytes
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Formalin fixed parrafin embedded mouse ovary tissue. Ab9021 used at 1:1000 incubated with overnight incubation with a biotinylated secondary and peroxidase/DAB detection.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (93)
ab9021 has been referenced in 93 publications.
- Gross A et al. Desmoplakin Maintains Transcellular Keratin Scaffolding and Protects From Intestinal Injury. Cell Mol Gastroenterol Hepatol 13:1181-1200 (2022). PubMed: 34929421
- Zhou WJ et al. Fructose-1,6-bisphosphate prevents pregnancy loss by inducing decidual COX-2+ macrophage differentiation. Sci Adv 8:eabj2488 (2022). PubMed: 35196096
- Wang LJ et al. Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation. Nat Commun 13:1626 (2022). PubMed: 35338152
- Mauduit O et al. A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands. Cell Rep 39:110663 (2022). PubMed: 35417692
- Shibata O et al. Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method. Mol Ther Nucleic Acids 28:342-352 (2022). PubMed: 35474735