Recombinant Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [rOV-TL12/30] to Cytokeratin 7
- Suitable for: Protein Array, IHC-P, WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 7 antibody [rOV-TL12/30]
See all Cytokeratin 7 primary antibodies -
Description
Mouse monoclonal [rOV-TL12/30] to Cytokeratin 7 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 7. (OTN 11, ovarian carcinoma cell line).
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Positive control
- IHC-P: Human endometrial carcinoma tissue; WB: HeLa cell lysate; Flow Cyt: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab218439 was purified by Protein A/G. -
Clonality
Monoclonal -
Clone number
rOV-TL12/30 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at room temperature. |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
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Flow Cyt |
Use 1-2µg for 106 cells.
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Notes |
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Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Primary incubation for 30 minutes at room temperature. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa. |
Flow Cyt
Use 1-2µg for 106 cells. |
Target
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Function
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
Tissue specificity
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3855 Human
- Omim: 148059 Human
- SwissProt: P08729 Human
- Unigene: 411501 Human
- Unigene: 670221 Human
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Alternative names
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human endometrial carcinoma tissue labeling Cytokeratin 7 with ab218439 at 1 µg/ml.
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Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 51 kDa -
Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 7 with ab218439 at 2 µg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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ab218439 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab218439 has not yet been referenced specifically in any publications.