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  1. Link

    cytokeratin-7-antibody-rov-tl1230-ab218439.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Recombinant

Recombinant Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)

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  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
  • Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
  • Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
  • Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
  • Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [rOV-TL12/30] to Cytokeratin 7
  • Suitable for: Protein Array, IHC-P, WB, Flow Cyt
  • Reacts with: Human

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Carrier Free

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Overview

  • Product name

    Anti-Cytokeratin 7 antibody [rOV-TL12/30]
    See all Cytokeratin 7 primary antibodies
  • Description

    Mouse monoclonal [rOV-TL12/30] to Cytokeratin 7
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Cytokeratin 7. (OTN 11, ovarian carcinoma cell line).

  • Positive control

    • IHC-P: Human endometrial carcinoma tissue; WB: HeLa cell lysate; Flow Cyt: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab218439 was purified by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    rOV-TL12/30
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP kits

Associated products

  • Alternative Versions

    • Anti-Cytokeratin 7 antibody [rOV-TL12/30] - BSA and Azide free (ab218900)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Cytokeratin 7 protein (ab132933)
  • Related Products

    • Recombinant Human Cytokeratin 7 protein (ab132933)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab218439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.

WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
Flow Cyt
Use 1-2µg for 106 cells.
Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.

WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
Flow Cyt
Use 1-2µg for 106 cells.

Target

  • Function

    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
  • Tissue specificity

    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P08729 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3855 Human
    • Omim: 148059 Human
    • SwissProt: P08729 Human
    • Unigene: 411501 Human
    • Unigene: 670221 Human
    • Alternative names

      • CK 7 antibody
      • CK-7 antibody
      • CK7 antibody
      • Cytokeratin 7 antibody
      • Cytokeratin-7 antibody
      • D15Wsu77e antibody
      • K2C7 antibody
      • K2C7_HUMAN antibody
      • K7 antibody
      • Keratin 7 antibody
      • Keratin 7, type II antibody
      • Keratin type II cytoskeletal 7 antibody
      • Keratin, 55K type II cytoskeletal antibody
      • Keratin, simple epithelial antibody
      • Keratin, simple epithelial type I, K7 antibody
      • Keratin, type II cytoskeletal 7 antibody
      • Keratin-7 antibody
      • Krt2-7 antibody
      • KRT7 antibody
      • MGC11625 antibody
      • MGC129731 antibody
      • MGC3625 antibody
      • Sarcolectin antibody
      • SCL antibody
      • Type II mesothelial keratin K7 antibody
      • Type-II keratin Kb7 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human endometrial carcinoma tissue labeling Cytokeratin 7 with ab218439 at 1 µg/ml.

    • Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
      Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
      Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Predicted band size: 51 kDa

    • Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
      Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)

      Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 7 with ab218439 at 2 µg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

    • Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
      Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
      ab218439 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
      Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab218439? Please let us know so that we can cite the reference in this datasheet.

    ab218439 has not yet been referenced specifically in any publications.

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