Anti-Cytokeratin 7 antibody [SPM270] (ab233903)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Cytokeratin 7 antibody [SPM270]
See all Cytokeratin 7 primary antibodies -
Description
Mouse monoclonal [SPM270] to Cytokeratin 7 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 7. OTN 11, ovarian carcinoma cell line.
-
Positive control
- IHC-P: Human lung small cell carcinoma tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
SPM270 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab233903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at room temperature. |
Target
-
Function
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
Tissue specificity
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 3855 Human
- Omim: 148059 Human
- SwissProt: P08729 Human
- Unigene: 411501 Human
- Unigene: 670221 Human
-
Alternative names
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SPM270] (ab233903)](https://www.abcam.com/ps/products/233/ab233903/Images/ab233903-308204-03.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SPM270] (ab233903)Formalin-fixed, paraffin-embedded human lung small cell carcinoma tissue stained for Cytokeratin 7 using ab233903 at 1 μg/ml in immunohistochemical analysis.
Datasheets and documents
References
ab233903 has not yet been referenced specifically in any publications.
