Overview

  • Product name

    Anti-Cytokeratin 7+17 antibody [C-46]
  • Description

    Mouse monoclonal [C-46] to Cytokeratin 7+17
  • Host species

    Mouse
  • Specificity

    Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa)
  • Tested applications

    Suitable for: ICC, WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Sheep, Cow, Dog, Human, Pig
    Does not react with: Mouse, Rat, Rabbit
  • Immunogen

    Tissue/ cell preparation (Human). (Cytoskeleton preparation of HeLa cells (cervix carcinoma)).

  • General notes

    Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from culture supernatant. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    C-46
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • Relevance

    Cytokeratin 7 and 17 is a heterotetramer of a type I and type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Cytokeratin 17 is required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • CK17 antibody
    • CK7 antibody
    • Cytokeratin 17 antibody
    • Cytokeratin 7 antibody
    • K17 antibody
    • K7 antibody
    • Keratin 17 antibody
    • Keratin 7 antibody
    • KRT17 antibody
    • KRT7 antibody
    see all

Images

  • Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.
  • Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/ml + HeLa cell lysate

    Observed band size: 49,51 kDa
    why is the actual band size different from the predicted?



    Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)
  • Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human breast by immunohistochemistry using formalin fixed, paraffin embedded tissue.

References

This product has been referenced in:

  • Sun Y  et al. Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis. Toxicology 262:162-70 (2009). Read more (PubMed: 19524636) »
  • Vojtesek B  et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma 37:333-42 (1990). Read more (PubMed: 1695327) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Nasal epithelium)
Permeabilization
No
Specification
Nasal epithelium
Fixative
heat (67C, 15 min)

Abcam user community

Verified customer

Submitted Aug 10 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Cow Tissue sections (Tongue)
Permeabilization
No
Specification
Tongue
Fixative
heat (67C, 15 min)

Abcam user community

Verified customer

Submitted Jun 11 2018

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