Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)


  • Product name
    Anti-Cytokeratin 8 + 18 + 19 antibody [2A4]
  • Description
    Mouse monoclonal [2A4] to Cytokeratin 8 + 18 + 19
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Cytokeratins isolated from cultured pig kidney epithelial cells.

  • General notes
    Balb/c spleen cells used to produce hybridoma.

    This antibody recognizes cytokeratins 8, 18 and 19 typical for simple epithelial cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    This antibody recognizes cytokeratins 8, 18 and 19 typical for simple epithelial cells.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab41825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab91353 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Relevance
    The cytokeratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. Keratin proteins belong to 2 families: acidic (or type I) and basic (or type II). The acidic keratins are coded by genes KRT9 to 19, the basic keratins by genes KRT1 to 8. Cytokeratins 8, 18 and 19 are encoded by the genes KRT8, 18 and 19 respectively. Type I and II keratins are usually coordinately synthesized in preferential pairs so that at least 1 member of each family is expressed in each epithelial cell, in equal proportions. Cytokeratin 8, together with its filament partner cytokeratin 18, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues. KRT8/18 mutations may predispose to liver disease, including cryptogenic cirrhosis, in humans. Unlike its related family members, cytokeratin 19 (the smallest known acidic cytokeratin at 40kDa) is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. It is often expressed in cultured epithelial cells and in some carcinomas.
  • Cellular localization
  • Database links
  • Alternative names
    • CK18 antibody
    • CK19 antibody
    • CK8 antibody
    • K18 antibody
    • K19 antibody
    • K8 antibody
    • keratin 18 antibody
    • keratin 19 antibody
    • keratin 8 antibody
    • Keratin type I 40kDa antibody
    • Keratin type I cytoskeletal 18 antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin type II cytoskeletal 8 antibody
    • KRT18 antibody
    • KRT19 antibody
    • KRT8 antibody
    see all


  • ab41825 staining Cytokeratin 8 + 18 + 19 in the MCF-7 epithelial cell line from Human mammary glands by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.3% in PBS and blocked with 5% serum for 8 hours at 4°C. Samples were incubated with primary antibody (10ug/ml) for 8 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(10ug/ml) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing MCF7 cells stained with ab41825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41825, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • IHC image of ab41825 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41825, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Zhou Q  et al. Abnormal expression of fibrosis markers, estrogen receptor a and stromal derived factor-1/chemokine (C-X-C motif) receptor-4 axis in intrauterine adhesions. Int J Mol Med 42:81-90 (2018). Read more (PubMed: 29568895) »
  • Le UT  et al. Intraoperative detection of circulating tumor cells in pulmonary venous blood during metastasectomy for colorectal lung metastases. Sci Rep 8:8751 (2018). ICC/IF ; Human . Read more (PubMed: 29884810) »

See all 8 Publications for this product

Customer reviews and Q&As

Vielen Dank für Ihren Anruf.

Leider sind 0,5% BSA das Maximum, dass das Labeling-Kit toleriert. Daher wäre entweder eine IP notwendig, mit der Sie den Antikörper von BSA aufreinigen notwendig, oder aber Sie benutzen ein weitere...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (MCF-7)
Yes - 0.3% Triton-X 100
Blocking step
Serum as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 24 2012

Thank you for contacting us.

While we do not provide explicit expiration dates for our antibodies, we will guaranteethemfor six months from the date of purchase when stored according to our datasheet instructions. For ab41825 and ab55138, we ...

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Expiration date: October 19th, 2012

I am very pleased to hear you would like to accept our offer and test ab41825 in ICC. This code will give you: 1 free primary antibody before the expiration date. To redeem this offe...

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Thank you for your enquiry regarding ab41825 and ab118855.

We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. These two products fall into the latte...

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