Key features and details
- Rabbit polyclonal to Cytokeratin 8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cytokeratin 8 antibody
See all Cytokeratin 8 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 8
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- HeLa cells; HUVEC and 293 cell extracts; Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab131514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cytokeratin 8 with ab131514 at 1/50 dilution (left) or ab131514 preincubated with blocking peptide (right).
Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cytokeratin 8 with ab131514 at 1/100 dilution.
All lanes : Anti-Cytokeratin 8 antibody (ab131514) at 1/500 dilution
Lane 1 : HUVEC extract
Lane 2 : 293 cell extract
Predicted band size: 53 kDa
ab131514 has not yet been referenced specifically in any publications.