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    cytokeratin-8-antibody-ab137855.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Anti-Cytokeratin 8 antibody (ab137855)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab137855)
  • Western blot - Anti-Cytokeratin 8 antibody (ab137855)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab137855)
  • Western blot - Anti-Cytokeratin 8 antibody (ab137855)

Key features and details

  • Rabbit polyclonal to Cytokeratin 8
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Cytokeratin 8 protein (ab156970)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cytokeratin 8 antibody
    See all Cytokeratin 8 primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-224 of Human Cytokeratin 8.

  • Positive control

    • 293T, A431, H1299, HeLaS3 and HepG2 cell lines Molt-4, Raji and mouse liver whole cell lysates; HeLa cells; A549 xenograft tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
    • Raji whole cell lysate (ab30124)
    • A431 whole cell lysate (ab30132)
    • A431 whole cell lysate (ab7909)
    • MOLT4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human Cytokeratin 8 protein (ab156970)

Applications

Our Abpromise guarantee covers the use of ab137855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in disease

    Cirrhosis
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Target information above from: UniProt accession P05787 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3856 Human
    • Entrez Gene: 16691 Mouse
    • Entrez Gene: 25626 Rat
    • Omim: 148060 Human
    • SwissProt: P05787 Human
    • SwissProt: P11679 Mouse
    • SwissProt: Q10758 Rat
    • Unigene: 533782 Human
    • Unigene: 708445 Human
    • Unigene: 358618 Mouse
    • Unigene: 11083 Rat
    see all
  • Alternative names

    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab137855)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab137855)
    Immunohistochemical analysis of parafin-embedded A549 xenograft tissue labelling Cytokeratin 8 with ab137855 at 1/500 dilution.
  • Western blot - Anti-Cytokeratin 8 antibody (ab137855)
    Western blot - Anti-Cytokeratin 8 antibody (ab137855)
    All lanes : Anti-Cytokeratin 8 antibody (ab137855) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab137855)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab137855)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling Cytokeratin 8 with ab137855 at 1/200 dilution. The image in the lower panel is merged with a DNA probe.
  • Western blot - Anti-Cytokeratin 8 antibody (ab137855)
    Western blot - Anti-Cytokeratin 8 antibody (ab137855)
    Anti-Cytokeratin 8 antibody (ab137855) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg

    Predicted band size: 53 kDa



    10% SDS PAGE

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab137855? Please let us know so that we can cite the reference in this datasheet.

    ab137855 has been referenced in 2 publications.

    • Li L  et al. Three-dimensional hepatocyte culture system for the study of Echinococcus multilocularis larval development. PLoS Negl Trop Dis 12:e0006309 (2018). PubMed: 29538424
    • Albaret MA  et al. Externalized Keratin 8: A Target at the Interface of Microenvironment and Intracellular Signaling in Colorectal Cancer Cells. Cancers (Basel) 10:N/A (2018). PubMed: 30453567

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab137855.
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