Anti-Cytokeratin 8 antibody (ab137855)
Key features and details
- Rabbit polyclonal to Cytokeratin 8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Cytokeratin 8 antibody
See all Cytokeratin 8 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-224 of Human Cytokeratin 8.
-
Positive control
- 293T, A431, H1299, HeLaS3 and HepG2 cell lines Molt-4, Raji and mouse liver whole cell lysates; HeLa cells; A549 xenograft tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 53 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
Involvement in disease
Cirrhosis -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
Cellular localization
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
-
Database links
- Entrez Gene: 3856 Human
- Entrez Gene: 16691 Mouse
- Entrez Gene: 25626 Rat
- Omim: 148060 Human
- SwissProt: P05787 Human
- SwissProt: P11679 Mouse
- SwissProt: Q10758 Rat
- Unigene: 533782 Human
see all -
Alternative names
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab137855)Immunohistochemical analysis of parafin-embedded A549 xenograft tissue labelling Cytokeratin 8 with ab137855 at 1/500 dilution.
-
Western blot - Anti-Cytokeratin 8 antibody (ab137855)All lanes : Anti-Cytokeratin 8 antibody (ab137855) at 1/1000 dilution
Lane 1 : MOLT4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab137855)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling Cytokeratin 8 with ab137855 at 1/200 dilution. The image in the lower panel is merged with a DNA probe. -
Western blot - Anti-Cytokeratin 8 antibody (ab137855)Anti-Cytokeratin 8 antibody (ab137855) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 53 kDa
10% SDS PAGE
Protocols
References (2)
ab137855 has been referenced in 2 publications.
- Li L et al. Three-dimensional hepatocyte culture system for the study of Echinococcus multilocularis larval development. PLoS Negl Trop Dis 12:e0006309 (2018). PubMed: 29538424
- Albaret MA et al. Externalized Keratin 8: A Target at the Interface of Microenvironment and Intracellular Signaling in Colorectal Cancer Cells. Cancers (Basel) 10:N/A (2018). PubMed: 30453567
