Anti-Cytokeratin 8 antibody (ab137855)

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Overview

  • Product name

    Anti-Cytokeratin 8 antibody
    See all Cytokeratin 8 primary antibodies

  • Description

    Rabbit polyclonal to Cytokeratin 8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-224 of Human Cytokeratin 8.

  • Positive control

    • 293T, A431, H1299, HeLaS3 and HepG2 cell lines Molt-4, Raji and mouse liver whole cell lysates; HeLa cells; A549 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

  • Tissue specificity

    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

  • Involvement in disease

    Cirrhosis

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

  • Cellular localization

    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab137855)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab137855)
    Immunohistochemical analysis of parafin-embedded A549 xenograft tissue labelling Cytokeratin 8 with ab137855 at 1/500 dilution.
  • Western blot - Anti-Cytokeratin 8 antibody (ab137855)
    Western blot - Anti-Cytokeratin 8 antibody (ab137855)
    All lanes : Anti-Cytokeratin 8 antibody (ab137855) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab137855)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab137855)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling Cytokeratin 8 with ab137855 at 1/200 dilution. The image in the lower panel is merged with a DNA probe.
  • Western blot - Anti-Cytokeratin 8 antibody (ab137855)
    Western blot - Anti-Cytokeratin 8 antibody (ab137855)
    Anti-Cytokeratin 8 antibody (ab137855) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg

    Predicted band size: 53 kDa



    10% SDS PAGE

References

This product has been referenced in:

  • Li L  et al. Three-dimensional hepatocyte culture system for the study of Echinococcus multilocularis larval development. PLoS Negl Trop Dis 12:e0006309 (2018). Read more (PubMed: 29538424) »
  • Albaret MA  et al. Externalized Keratin 8: A Target at the Interface of Microenvironment and Intracellular Signaling in Colorectal Cancer Cells. Cancers (Basel) 10:N/A (2018). Read more (PubMed: 30453567) »
See all 2 Publications for this product

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