Overview

  • Product name

    Anti-Cytokeratin 8 antibody
    See all Cytokeratin 8 primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A recombinant fragment corresponding to a region within amino acids 227- 483 of Human Cytokeratin 8 (Uniprot ID#P05787).

  • Positive control

    • HeLa, HepG2 and JC whole cell lysates. Human gastric carcinoma tissue. HeLa cells.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in disease

    Cirrhosis
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Images

  • All lanes : Anti-Cytokeratin 8 antibody (ab154301) at 1/20000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Cytokeratin 8 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 53 kDa

  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma labeling Cytokeratin 8 using ab154301 at 1/500 dilution.
  • Anti-Cytokeratin 8 antibody (ab154301) at 1/10000 dilution + JC cell lysate at 30 µg

    Predicted band size: 53 kDa



    10% SDS PAGE
  • All lanes : Anti-Cytokeratin 8 antibody (ab154301) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Cytokeratin 8 using ab154301 at 1/500 dilution. Right panel costained with Hoechst 33342.

References

This product has been referenced in:

  • Johnson JR  et al. Microarray and pathway analysis of two COMMA-Dß derived clones reveal important differences relevant to their developmental capacity in-vivo. Oncotarget 10:2118-2135 (2019). Read more (PubMed: 31040905) »
See 1 Publication for this product

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