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    cytokeratin-8-antibody-ab154301.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Anti-Cytokeratin 8 antibody (ab154301)

  • Datasheet
  • SDS
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Western blot - Anti-Cytokeratin 8 antibody (ab154301)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab154301)
  • Western blot - Anti-Cytokeratin 8 antibody (ab154301)
  • Western blot - Anti-Cytokeratin 8 antibody (ab154301)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab154301)

Key features and details

  • Rabbit polyclonal to Cytokeratin 8
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Cytokeratin 8 protein (ab156970)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cytokeratin 8 antibody
    See all Cytokeratin 8 primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A recombinant fragment corresponding to a region within amino acids 227- 483 of Human Cytokeratin 8 (Uniprot ID#P05787).

  • Positive control

    • HeLa, HepG2 and JC whole cell lysates. Human gastric carcinoma tissue. HeLa cells.
  • General notes

    Keep as concentrated solution.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Cytokeratin 8 protein (ab156970)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/5000 - 1/20000. Predicted molecular weight: 53 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 53 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in disease

    Cirrhosis
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Target information above from: UniProt accession P05787 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3856 Human
    • Entrez Gene: 16691 Mouse
    • Omim: 148060 Human
    • SwissProt: P05787 Human
    • SwissProt: P11679 Mouse
    • Unigene: 533782 Human
    • Unigene: 708445 Human
    • Unigene: 358618 Mouse
    • Alternative names

      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Images

    • Western blot - Anti-Cytokeratin 8 antibody (ab154301)
      Western blot - Anti-Cytokeratin 8 antibody (ab154301)
      All lanes : Anti-Cytokeratin 8 antibody (ab154301) at 1/20000 dilution

      Lane 1 : WT HeLa cell extract
      Lane 2 : Cytokeratin 8 Knockout HeLa cell extracts

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG

      Predicted band size: 53 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab154301)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab154301)
      Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma labeling Cytokeratin 8 using ab154301 at 1/500 dilution.
    • Western blot - Anti-Cytokeratin 8 antibody (ab154301)
      Western blot - Anti-Cytokeratin 8 antibody (ab154301)
      Anti-Cytokeratin 8 antibody (ab154301) at 1/10000 dilution + JC cell lysate at 30 µg

      Predicted band size: 53 kDa



      10% SDS PAGE
    • Western blot - Anti-Cytokeratin 8 antibody (ab154301)
      Western blot - Anti-Cytokeratin 8 antibody (ab154301)
      All lanes : Anti-Cytokeratin 8 antibody (ab154301) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 53 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab154301)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab154301)
      Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Cytokeratin 8 using ab154301 at 1/500 dilution. Right panel costained with Hoechst 33342.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab154301? Please let us know so that we can cite the reference in this datasheet.

    ab154301 has been referenced in 2 publications.

    • Johnson JR  et al. Microarray and pathway analysis of two COMMA-Dß derived clones reveal important differences relevant to their developmental capacity in-vivo. Oncotarget 10:2118-2135 (2019). PubMed: 31040905
    • Pavlíková N  et al. Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p'-DDT and p,p'-DDE. Sci Rep 9:18026 (2019). PubMed: 31792309

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Cytokeratin 8 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (MDCK (Madin-Darby Canine Kidney) cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    With non specific shRNA or Keratin 8 shRNA
    Specification
    MDCK (Madin-Darby Canine Kidney) cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 30 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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