Key features and details
- Rabbit polyclonal to Cytokeratin 8
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Cytokeratin 8 antibody
See all Cytokeratin 8 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 8
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human Cytokeratin 8 aa 1-483.
MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGL GGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNN KFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQ LETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDV DEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDN SRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDL RRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDAN AKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEES RLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSS SFSRTSSSRAVVVKKIETRDGKLVSESSDV LPK
Database link: P05787
- 293T, HeLa, DU145 and NIH3T3 cell line lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 66 kDa.|
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling Cytokeratin 8 with ab175249 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Cytokeratin 8 with ab175249 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175249. Blue DAPI for nuclear staining.
All lanes : Anti-Cytokeratin 8 antibody (ab175249) at 1/500 dilution
Lane 1 : 293T cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : DU145 cell line lysate
Lane 4 : NIH3T3 cell line lysate
Predicted band size: 66 kDa
ab175249 has not yet been referenced specifically in any publications.