Overview

  • Product name

    Anti-Cytokeratin 8 antibody
    See all Cytokeratin 8 primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish Cytokeratin 8 aa 93-345. The exact sequence is proprietary.
    Database link: Q6NWF6

  • Positive control

    • Adult zebrafish tissue; zebrafish eye and whole animal cell lysate
  • General notes

    References regarding use as Epidermal marker in embryonic, mature zebrafish and regeneration:

    Imboden M  et al. Cytokeratin 8 is a suitable epidermal marker during zebrafish development. C R Acad Sci III 320:689-700 (1997). Zebrafish . Read more (PubMed: 9377174)

    Martorana ML  et al. Zebrafish keratin 8 is expressed at high levels in the epidermis of regenerating caudal fin. Int J Dev Biol 45:449-52 (2001). Zebrafish . Read more (PubMed: 11330866) »

     

     

Properties

Applications

Our Abpromise guarantee covers the use of ab210106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.

Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.

Target

  • Function

    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in disease

    Cirrhosis
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • Database links

    • Alternative names

      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Images

    • All lanes : Anti-Cytokeratin 8 antibody (ab210106) at 1/1000 dilution

      Lane 1 : Zebrafish whole cell lysate
      Lane 2 : Zebrafish eye whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 53 kDa



      7.5% SDS PAGE

    • Immunohistochemical analysis of zebrafish tissue labeling Cytokeratin 8 with ab210106 at 1/300 dilution. 

    References

    ab210106 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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