Key features and details
- Rabbit polyclonal to Cytokeratin 8
- Suitable for: IHC-P, WB
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-Cytokeratin 8 antibody
See all Cytokeratin 8 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 8
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Zebrafish
Recombinant fragment within Zebrafish Cytokeratin 8 aa 93-345. The exact sequence is proprietary.
Database link: Q6NWF6
- Adult zebrafish tissue; zebrafish eye and whole animal cell lysate
References regarding use as Epidermal marker in embryonic, mature zebrafish and regeneration:
Imboden M et al. Cytokeratin 8 is a suitable epidermal marker during zebrafish development. C R Acad Sci III 320:689-700 (1997). Zebrafish . Read more (PubMed: 9377174)
Martorana ML et al. Zebrafish keratin 8 is expressed at high levels in the epidermis of regenerating caudal fin. Int J Dev Biol 45:449-52 (2001). Zebrafish . Read more (PubMed: 11330866) »
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab210106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.
Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
|WB||1/500 - 1/3000. Predicted molecular weight: 53 kDa.|
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
All lanes : Anti-Cytokeratin 8 antibody (ab210106) at 1/1000 dilution
Lane 1 : Zebrafish whole cell lysate
Lane 2 : Zebrafish eye whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
7.5% SDS PAGE
Immunohistochemical analysis of zebrafish tissue labeling Cytokeratin 8 with ab210106 at 1/300 dilution.
ab210106 has not yet been referenced specifically in any publications.