Key features and details
- Rabbit polyclonal to Cytokeratin 8
- Suitable for: ICC/IF, IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Cytokeratin 8 antibody
See all Cytokeratin 8 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 8
Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic non-phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab59400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
ab59400 staining Cytokeratin 8 in c57b6 mouse prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citric acid. Samples were then blocked using 3% BSA for 1 hour at 25°C and then incubated with ab59400 at a 1/100 dilution for 18 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.
ICC/IF image of ab59400 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59400, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab59400 staining Cytokeratin 8 in Human breast cancer cells xenografted into nude mice by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formalin and subjected to heat-mediated antigen retrieval in citrate buffer (0.1M Sodium Citrate) prior to blocking with 5% serum for 1 hour at 4°C. The primary antibody was diluted 1/200 in 5% goat serum in PBS and incubated with the sample for 12 hours at 4°C. A Biotin-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000.
ab59400 staining Cytokeratin 8 in murine mammary gland duct tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA for 1 hour at 23°C and then incubated with ab59400 at a 1/250 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 568 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59400 at 1/50 dilution, in the presence (right) and absence (left) of immunising peptide. Then, a polymer secondary antibody was used for detection.
All lanes : Anti-Cytokeratin 8 antibody (ab59400) at 1/500 dilution
Lane 1 : EGF treated (200ng/ml, 30mins) 293 cell extracts
Lane 2 : EGF treated (200ng/ml, 30mins) 293 cell extracts with immunising peptide
Predicted band size: 54 kDa
Observed band size: 54 kDa
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells, labeling Cytokeratin 8 with ab59400. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Cytokeratin 8 with ab59400 at 1/100 dilution. Tissue sections were fixed with formaldehyde; heat mediated antigen retrieval was performed using a citric acid. 2% BSA was used to block, followed by incubation with ab59400 in TBS/BSA/azide for 2 hours at 21°C. a polyclonal goat anti-rabbit IgG bitin conjugated secondary antibody was used at 1/300 dilution.
ab59400 has been referenced in 29 publications.
- Beauboeuf R et al. Alterations in the gustatory papillae after anterior bite plate insertion in growing rats. J Orthod Sci 8:4 (2019). PubMed: 31001496
- Gao Y et al. Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice. Biol Res 52:30 (2019). PubMed: 31088536
- Gong X et al. Expression of ILK in renal stroma is essential for multiple aspects of renal development. Am J Physiol Renal Physiol 315:F374-F385 (2018). PubMed: 29638158
- Alamri AM et al. Expanding primary cells from mucoepidermoid and other salivary gland neoplasms for genetic and chemosensitivity testing. Dis Model Mech 11:N/A (2018). PubMed: 29419396
- Wang X et al. Therapeutic effects of conditioned medium from bone marrow-derived mesenchymal stem cells on epithelial-mesenchymal transition in A549 cells. Int J Mol Med 41:659-668 (2018). WB . PubMed: 29207055