Recombinant Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488) (ab192467)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1628Y] to Cytokeratin 8 (Alexa Fluor® 488)
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488)
See all Cytokeratin 8 primary antibodies -
Description
Rabbit monoclonal [EP1628Y] to Cytokeratin 8 (Alexa Fluor® 488) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Cytokeratin 8 aa 300-400 (C terminal). The exact sequence is proprietary.
Database link: P05787 -
Positive control
- ICC/IF: HeLa cells Flow Cyt: HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Dissociation constant (KD)
KD = 4.60 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP1628Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 647) (ab192468)
- Anti-Cytokeratin 8 antibody [EP1628Y] (HRP) (ab193094)
- Anti-Cytokeratin 8 antibody [EP1628Y] (PE) (ab209297)
- Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 405) (ab210139)
- Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free (ab217173)
- Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab192467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/100. | |
| Flow Cyt | 1/500. |
Target
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Function
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
Involvement in disease
Cirrhosis -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
Cellular localization
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
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Database links
- Entrez Gene: 3856 Human
- Entrez Gene: 16691 Mouse
- Omim: 148060 Human
- SwissProt: P05787 Human
- SwissProt: P11679 Mouse
- Unigene: 533782 Human
- Unigene: 708445 Human
- Unigene: 358618 Mouse
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Alternative names
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
Images
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![Immunocytochemistry - Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488) (ab192467)](https://www.abcam.com/ps/products/192/ab192467/Images/ab192467-232993-anti-cytokeratin-8-antibody-ep1628y-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry - Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488) (ab192467)ab192467 staining Cytokeratin 8 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192647 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) (ab150120) at 2 μg/ml (shown in pseudo-color red).
Nuclear DNA was labeled in blue with DAPI.
This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)
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![Flow Cytometry - Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488) (ab192467)](https://www.abcam.com/ps/products/192/ab192467/Images/ab192467-256990-anti-cytokeratin-8-antibody-ep1628y-alexa-fluor-488-flow-cytometry.jpg)
Flow Cytometry - Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488) (ab192467)Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab192467 (red line).
The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192467, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunocytochemistry - Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488) (ab192467)](https://www.abcam.com/ps/products/192/ab192467/Images/ab192467-397246-anti-cytokeratin-8-antibody-ep1628y-alexa-fluor-488-immunocytochemistry-mammary-human.jpg)
Immunocytochemistry - Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488) (ab192467)Rosenbluth J et al., Nat Commun, 11(1), 1711. Fig 1c.; doi: 10.1038/s41467-020-15548-7. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Immunocytochemistry/immunofluorescence analysis of human mammary organoids grown on chamber slides labelling Cytokeratin 8 with ab192467 (green), Cytokeratin 14 with ab206100 (red), and Actin (white). Cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature and permeabilized with 0.5% Triton X-100 for 10 minutes at 4 °C. Primary antibodies incubated overnight at 4 °C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 µm. Organoids were imaged by confocal microscopy.
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![Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488) (ab192467)](https://www.abcam.com/ps/products/192/ab192467/Images/ab192467-6-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 8 antibody [EP1628Y] (Alexa Fluor® 488) (ab192467)
Protocols
References (4)
ab192467 has been referenced in 4 publications.
- Rosenbluth JM et al. Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages. Nat Commun 11:1711 (2020). ICC . PubMed: 32249764
- Deuse T et al. Hypoimmunogenic derivatives of induced pluripotent stem cells evade immune rejection in fully immunocompetent allogeneic recipients. Nat Biotechnol 37:252-258 (2019). IF ; Mouse . PubMed: 30778232
- Crowell PD et al. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell Rep 28:1499-1510.e6 (2019). Flow Cyt ; Mouse . PubMed: 31390564
- Barros-Silva JD et al. Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer. Cell Rep 25:3504-3518.e6 (2018). IF ; Mouse . PubMed: 30566873
