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  1. Link

    cytokeratin-8-antibody-ep1628y-cytoskeleton-marker-ab53280.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

  • Datasheet
  • SDS
Reviews (8)Q&A (1)References (80)

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Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunoprecipitation - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • OI-RD Scanning - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1628Y] to Cytokeratin 8 - Cytoskeleton Marker
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP PE

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Overview

  • Product name

    Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker
    See all Cytokeratin 8 primary antibodies
  • Description

    Rabbit monoclonal [EP1628Y] to Cytokeratin 8 - Cytoskeleton Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast adenocarcinona, ovarian carcinoma, breast carcinoima, colon adenocarcinoma, endometrial carcinoma and thyroid carcinoma tissue; mouse liver tissue; ICC/IF: HT-29 and HeLa cells; WB: HeLa, A431 and HaCaT cell lysates; Human breast cancer lysates and Mouse colon lysate; Flow Cyt (intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 4.60 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1628Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)
    • Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)
    • HRP Anti-Cytokeratin 8 antibody [EP1628Y] (ab193094)
    • PE Anti-Cytokeratin 8 antibody [EP1628Y] (ab209297)
    • Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y] (ab210139)
    • Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free (ab217173)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human KRT8 (Cytokeratin 8) knockout HeLa cell line (ab255400)
  • KO cell lysates

    • Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (ab263785)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Cytokeratin 8 protein (ab156970)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).

For unpurified use at 1/25,000 - 1/50,000.

IP
1/20.

For unpurified use at 1:70.

IHC-P (3)
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF (2)
1/100 - 1/500.
Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).

For unpurified use at 1/25,000 - 1/50,000.

IP
1/20.

For unpurified use at 1:70.

IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/500.

Target

  • Function

    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in disease

    Cirrhosis
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Target information above from: UniProt accession P05787 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3856 Human
    • Entrez Gene: 16691 Mouse
    • Entrez Gene: 25626 Rat
    • Omim: 148060 Human
    • SwissProt: P05787 Human
    • SwissProt: P11679 Mouse
    • SwissProt: Q10758 Rat
    • Unigene: 533782 Human
    • Unigene: 708445 Human
    • Unigene: 358618 Mouse
    • Unigene: 11083 Rat
    see all
  • Alternative names

    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Images

  • Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    All lanes : Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280) at 1/10000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Wild-type HeLa cell lysate
    Lane 4 : KRT8 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 54 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab53280 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.  

     ab53280 was shown to react with Cytokeratin 8 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255400 (knockout cell lysate ab263785) was used. Wild-type and Cytokeratin 8 knockout samples were subjected to SDS-PAGE. ab53280 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 (For unpurified use at 1/25,000 - 1/50,000) dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Immunocytochemistry/ Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Cytokeratin 9 with Purified ab53280 at 1:500 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    Alexa Fluor® 488 (ab192467) and Alexa Fluor® 647 (ab192468) conjugated versions are available for this clone.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Cytokeratin 8 with Purified ab53280 at 1:250 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 8 with purified ab53280 at 1/20 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

    Alexa Fluorr®488 (ab192467) and Alexa Fluorr®647 (ab192468) conjugated versions are available for this clone. 

     

     

  • Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    All lanes : Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280) at 1/10000 dilution (purified)

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
    Lane 2 : Human breast cancer lysates
    Lane 3 : HaCaT (Human skin keratinocyte) whole cell lysates
    Lane 4 : Mouse colon lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 54 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunoprecipitation - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    ab53280 (purified) at 1:20 dilution (0.2μg) immunoprecipitating Cytokeratin 8 in HeLa whole cell lysate.

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, 10μg
    Lane 2 (+): ab53280 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab53280 in HeLa whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)Stewart, M.K. et al PLoS One. 2016 Apr 21;11(4):e0154162. doi: 10.1371/journal.pone.0154162. eCollection 2016 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Panx1-/- mice have normal mammary gland epithelial differentiation at lactation

    Immunofluorescent analysis of luminal epithelial marker keratin 8 (green) and myoepithelial marker keratin14 (red) revealed a similar staining pattern in Panx1-/- mice compared to control mice during lactation. Paraffin-embedded tissue samples.

    Hoescht (blue) denotes nuclei. N = 6. Scale bars = 50 um. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling Cytokeratin 8 with purified ab53280 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Unpurified ab53280 (1:250) staining human Cyotkeratin 8 in human breast adenocarcinoma tissue by immunohistochemistry using paraffin embedded tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Cytokeratin 8 with Purified ab53280 at 1:250 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280) at 1/50000 dilution (unpurified) + A431 cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)This image is courtesy of an anonymous Abreview

    Unpurified ab53280 staining Cytokeratin 8 in Mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 10% serum for 20 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/75 in TBS + 1% BSA) for 1 hour at 23°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Immunofluorescent staining of HeLa cells using unpurified ab53280 (1:100).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)This image is courtesy of Dr. Shaohua Li

    Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

    Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    Fix in 3%PFA in PBS for 30 min at RTIncubate in 7.5% sucrose-PBS for 3h at RTIncubate in 15% sucrose-PBS at 4 degree Celsius overnightEmbed the EBs in tissue-Tek OCT compoundCut frozen sections to 4-20 µm thickness

    Primary antibody 1: Rabbit anti cytokeratin 8 (unpurified ab53280), 1:100

    Primary antibody 2: Rat anti-perlecan, 1:100
    Secondary antibody 1: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) pre-adsorbed (ab150081), 1:200

    Secondary antibody 2: Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5®) pre-adsorbed (ab150081), 1:200
    Nuclei were counterstained with DAPI

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Fluorescent immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using unpurified ab53280. Green-CK8 red-PI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using unpurified ab53280. Green-CK8 red-PI

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Fluorescent immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using unpurified ab53280. Green-CK8 red-PI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Fluorescent immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue using unpurified ab53280. Green-CK8 red-PI.

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

    Overlay histogram showingHeLa cells stained with unpurifiedab53280 (red line). The cells were fixed with 2%PFA (room temperature, 30 min) and then permeabilized with 1%FACS permeabilizing solutionfor 30 min. The cells were then incubated in3% FBS in 1X PBSfollowed by the antibody (ab53280, 1/20 dilution) for1 hourat room temperature. The cells were then incubated for 30 min at room temperature with the secondary antibody. An isotype control antibody (black line) was used and an unlabelled sample (blue line) was also used as a control. 

     

     

  • OI-RD Scanning - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    OI-RD Scanning - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
    Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (80)

Publishing research using ab53280? Please let us know so that we can cite the reference in this datasheet.

ab53280 has been referenced in 80 publications.

  • Gao H  et al. Characterization of murine mammary stem/progenitor cells in a D-galactose-induced aging model. Aging (Albany NY) 13:11762-11773 (2021). PubMed: 33878032
  • Ryan FJ  et al. Zika Virus Infection of Human Iris Pigment Epithelial Cells. Front Immunol 12:644153 (2021). PubMed: 33968035
  • Cai J  et al. Exosomes in ovarian cancer ascites promote epithelial-mesenchymal transition of ovarian cancer cells by delivery of miR-6780b-5p. Cell Death Dis 12:210 (2021). PubMed: 33627627
  • Gu VW  et al. AP-2? Is Required for Maintenance of Multipotent Mammary Stem Cells. Stem Cell Reports 16:106-119 (2021). PubMed: 33382976
  • Lang C  et al. m6 A modification of lncRNA PCAT6 promotes bone metastasis in prostate cancer through IGF2BP2-mediated IGF1R mRNA stabilization. Clin Transl Med 11:e426 (2021). PubMed: 34185427
View all Publications for this product

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1-8 of 8 Abreviews

IHC - Wholemount abreview for Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Cornea)
Specification
Cornea
Read More

MRS. Aya Amitai Lange

Verified customer

Submitted Dec 13 2019

Western blot abreview for Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (CWR-R1 Prostate cancer cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
CWR-R1 Prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Jul 05 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8 antibody [EP1628Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC3 Prostate cancer cells)
Permeabilization
Yes - 0.1% Triton X-100
Specification
PC3 Prostate cancer cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

DR. Dimitra Kalamida

Verified customer

Submitted Mar 22 2019

IHC - Wholemount abreview for Anti-Cytokeratin 8 antibody [EP1628Y]

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Mammary Gland from a WT mouse)
Specification
Mammary Gland from a WT mouse
Read More

DR. Francesco Elia Marino

Verified customer

Submitted Oct 03 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 8 antibody [EP1628Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate & Urethra)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
No
Specification
Prostate & Urethra
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 01 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 8 antibody [EP1628Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (Oviduct, epithelial cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization
No
Specification
Oviduct, epithelial cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 27 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8 antibody [EP1628Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (Differentiated P19 cell line)
Specification
Differentiated P19 cell line
Permeabilization
Yes - Triton X-100
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 24 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 8 antibody [EP1628Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
Read More

Abcam user community

Verified customer

Submitted Mar 04 2013

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