IHC - Wholemount abreview for Anti-Cytokeratin 8 antibody [EP1628Y]

Excellent
Abreviews
Application
IHC - Wholemount
Sample
Mouse Tissue (Mammary Gland from a WT mouse)
Specification
Mammary Gland from a WT mouse

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in CAS Block
Dilution
1/400

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa 647 Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647

Additional data

Additional Notes
This antibody performs very well. The optimal antigen retrieval condition in my experience is 10mM Sodium Citrate Buffer 0.05% Tween (Tween addition is optional) pH 6.0 - An enzymatic antigen retrieval option with Proteinase K is also suitable but you need to be careful with the concentration. Too much could easily damage your tissue. The attached picture shows a mammary duct (from a WT mouse) stained for CK8 (in yellow) and Hoechst (as counterstain). The picture was taken using a Leica Microscope and using a 20x Objective.

Dr. Francesco Elia Marino

Verified customer

Submitted Oct 03 2018

Sign up