Western blot abreview for Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (CWR-R1 Prostate cancer cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
CWR-R1 Prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
1 minute(s) and 43 second(s)
Bands
Specific: 52 kDa

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Submitted Jul 05 2019

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