Overview

  • Product name

    Anti-Cytokeratin 8 antibody [K8.8]
    See all Cytokeratin 8 primary antibodies
  • Description

    Mouse monoclonal [K8.8] to Cytokeratin 8
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, IP, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Full length native protein (purified) corresponding to Human Cytokeratin 8. Keratin preparation from a human carcinoma
    Database link: P05787

  • Positive control

    • MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab193346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at 2-4 µg/mg of lysate.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).

Target

  • Function

    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in disease

    Cirrhosis
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed paraffin embedded human colon carcinoma labeling Cytokeratin 8 with ab193346 at 0.5 μg/ml

References

ab193346 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab193346.
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