Key features and details
- Mouse monoclonal [K8.8] to Cytokeratin 8
- Suitable for: ICC/IF, IHC-P, Flow Cyt, IP, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Cytokeratin 8 antibody [K8.8]
See all Cytokeratin 8 primary antibodies
DescriptionMouse monoclonal [K8.8] to Cytokeratin 8
Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cyt, IP, IHC-Fr, WBmore details
Species reactivityReacts with: Human
Does not react with: Rat
Full length native protein (purified) corresponding to Human Cytokeratin 8. Keratin preparation from a human carcinoma
Database link: P05787
- MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab193346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at 2-4 µg/mg of lysate.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).|
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
ab193346 has not yet been referenced specifically in any publications.