Western blot abreview for Anti-Cytokeratin 8 antibody [M20]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer cell line)
Loading amount
20 µg
Specification
pancreatic cancer cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBS-Tween

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 53 kDa

Additional data

PubMed ID
17875703: View

Dr. Hannah Whiteman

Verified customer

Submitted Jul 10 2008

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