MCF-7 cells were grown in 8 well chamber slides, fixed with Acetone (-20C) for 20 minutes, permeabilised with 0.1% Triton-X-100 for 5 minutes at 4C and blocked with 1% Fish gelatin for 1 hour at RT. Primary antibody was diluted to 1/100 in 0.1% BSA and Alexa Fluor 488 was diluted 1/1000, also in 0.1% BSA. Slides were mounted using mounting medium containing DAPI.
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Submitted Mar 14 2008
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