Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8 antibody [M20]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF-7 breast cancer cells)
Specification
MCF-7 breast cancer cells
Fixative
Acetone
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Fish Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
MCF-7 cells were grown in 8 well chamber slides, fixed with Acetone (-20C) for 20 minutes, permeabilised with 0.1% Triton-X-100 for 5 minutes at 4C and blocked with 1% Fish gelatin for 1 hour at RT. Primary antibody was diluted to 1/100 in 0.1% BSA and Alexa Fluor 488 was diluted 1/1000, also in 0.1% BSA. Slides were mounted using mounting medium containing DAPI.

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Submitted Mar 14 2008

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