Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8 antibody [M20]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate)
Specification
prostate
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-X
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Other product details

Dilution
1/100
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA/PBS

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-mouse AlexaFluor 488 *highly conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
4% PFA for 10 min at RT

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Submitted Oct 21 2009

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