Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cancer cell lines HCT116/HT29/Caco2)
Loading amount
40 µg
Specification
Colon cancer cell lines HCT116/HT29/Caco2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Sigma
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: Doublet at 40 and 50 kDa
Additional data
Additional Notes
Doublet seen between 40 and 50 kDa. Works well on fractionated cells and whole cell lysate. Can be frozen in milk solution and reused multiple times.
16 hour incubation at 4C. Unable to achieve positive results using antibody for IP.
Image exposure time for Gel imager not film.
16 hour incubation at 4C. Unable to achieve positive results using antibody for IP.
Image exposure time for Gel imager not film.
Abcam user community
Verified customer
Submitted Sep 18 2006