For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cytokeratin-8-antibody-m20-cytoskeleton-marker-ab9023.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
Share by email
Validated using a knockout cell line

Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)

  • Datasheet
  • SDS
Reviews (10)Q&A (4)References (24)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
  • Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
  • Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
  • Flow Cytometry - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)

Key features and details

  • Mouse monoclonal [M20] to Cytokeratin 8 - Cytoskeleton Marker
  • Suitable for: Flow Cyt, IHC-P, IHC-Fr, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human Cytokeratin 8 protein (ab156970)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Primary
Product image
Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

View more associated products

Overview

  • Product name

    Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker
    See all Cytokeratin 8 primary antibodies
  • Description

    Mouse monoclonal [M20] to Cytokeratin 8 - Cytoskeleton Marker
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Keratin isolated from the human breast carcinoma cell line MCF-7.

  • Positive control

    • WB: HeLa, A431, PC3 and LNCaP cell lysates. ICC/IF: MCF-7, pancreatic cancer and epithelial ovarian cancer cells. IHC-Fr: Human colon tissue.
  • General notes

    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.3
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% Fetal calf serum
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Primary antibody notes

    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
  • Clonality

    Monoclonal
  • Clone number

    M20
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • KO cell lines

    • Human KRT8 (Cytokeratin 8) knockout HeLa cell line (ab255400)
  • KO cell lysates

    • Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (ab263785)
  • Recombinant Protein

    • Recombinant Human Cytokeratin 8 protein (ab156970)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-Fr
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
Flow Cyt
Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P (1)
Use a concentration of 4 µg/ml.
IHC-Fr
1/100 - 1/200. For human colon 20min ice cold acetone fixation was carried out with 60min incubation with ab9023 at 37C.
WB (4)
1/100 - 1/1000.
Notes
Flow Cyt
Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 4 µg/ml.
IHC-Fr
1/100 - 1/200. For human colon 20min ice cold acetone fixation was carried out with 60min incubation with ab9023 at 37C.
WB
1/100 - 1/1000.

Target

  • Function

    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in disease

    Cirrhosis
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Target information above from: UniProt accession P05787 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3856 Human
    • Omim: 148060 Human
    • SwissProt: P05787 Human
    • Unigene: 533782 Human
    • Unigene: 708445 Human
    • Alternative names

      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Images

    • Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      All lanes : Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)

      Lane 1 : A431 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : Wild-type HeLa cell lysate
      Lane 4 : KRT8 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.


      Lanes 1 - 4: Merged signal (red and green). Green - ab9023 observed at 55 kDa. Red - loading control, ab181602 observed at 37 kDa.  

       ab9023 was shown to react with Cytokeratin 8 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255400 (knockout cell lysate ab263785) was used. Wild-type and Cytokeratin 8 knockout samples were subjected to SDS-PAGE. ab9023 and Anti-GAPDH antibody EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)

      The image shows different concentration of total protein from normal and tumour colon tissues.

      This picture was kindly supplied as part of the review submitted by Semona Rupchand.

      The image shows different concentration of total protein from normal and tumour colon tissues.

      This picture was kindly supplied as part of the review submitted by Semona Rupchand.

    • Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)This image is a courtesy of Anonymous Abreview
      All lanes : Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023) at 1/2000 dilution

      Lane 1 : Lysate prepared from human prostate cancer LNCaP cells
      Lane 2 : Lysate prepared from human prostate cancer PC3 cells

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat polyclonal to mouse IgG at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 50 kDa why is the actual band size different from the predicted?
      Additional bands at: 26 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)

      Immunohistochemistry analysis of frozen section of human colon labeling Cytokeratin 8 with ab9023.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Immunohistochemistry on paraffin section of human colon
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Ab9023 staining human normal liver parenchima tissue. Staining is localized to cell membrane.
      Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Flow Cytometry - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Flow Cytometry - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)

      Overlay histogram showing MCF-7 cells stained with ab9023 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.5% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9023, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/250 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.5% PBS-Tween used under the same conditions.

    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (ab9023)
      Immunohistochemistry on frozen section of human colon

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (24)

    Publishing research using ab9023? Please let us know so that we can cite the reference in this datasheet.

    ab9023 has been referenced in 24 publications.

    • Cao J  et al. Estrogen attenuates TGF-ß1-induced EMT in intrauterine adhesion by activating Wnt/ß-catenin signaling pathway. Braz J Med Biol Res 53:e9794 (2020). PubMed: 32638833
    • Ehsani E  et al. Improved differentiation of human enriched CD133+CD24+ renal progenitor cells derived from embryonic stem cell with embryonic mouse kidney-derived mesenchymal stem cells co-culture. Differentiation 109:1-8 (2019). PubMed: 31323479
    • Nguyen QH  et al. Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity. Nat Commun 9:2028 (2018). PubMed: 29795293
    • Baek A  et al. Autophagy and KRT8/keratin 8 protect degeneration of retinal pigment epithelium under oxidative stress. Autophagy 13:248-263 (2017). PubMed: 28045574
    • McCormick NH  et al. ZnT4 (SLC30A4)-null ("lethal milk") mice have defects in mammary gland secretion and hallmarks of precocious involution during lactation. Am J Physiol Regul Integr Comp Physiol 310:R33-40 (2016). WB ; Mouse . PubMed: 26538236
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 14 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rabbit Cell (Primary rabbit intestinal epithelial cells)
    Permeabilization
    Yes - 0.25% Triton X-100
    Specification
    Primary rabbit intestinal epithelial cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Egi Kardia

    Verified customer

    Submitted Dec 04 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF-7 Cells)
    Permeabilization
    Yes - 0.25% Triton X-100
    Specification
    MCF-7 Cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 22 2015

    Question

    Phone call requesting free sample of ab9023.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2011

    Answer

    Thank you for contacting us. I am sorry that the ab14053 is currently out of stock. Having looked into the suitability of ab9023 as a replacement I would now not recommend its use. Although some of our customers have used it successfully in mouse, we have also had several reports of it producing non-specific staining. I would therefore suggest and antibody such as ab59400 which has been tested with mouse samples and with ELISA, immunocytochemistry, immunohistochemistry and westernblotting. This is a rabbit polyclonal and is currently in stock. This antibody is covered by the Abpromise for the application and species you intend to use, it would therefore not qualify for a testing discount. This does however mean that if you are not satisfied with the results and we are not able to help you improve them, you would be eligable for a free replacement (or your choice) or a refund. If there is an alternative antibody that you prefer which has not been tested in mouse or for your application then I would be able to issue the testing discount.  If you feel the replacement suggested is not suitable replacement please could you give me the full details of what experiments you intend to do and I can see which antibody may match your criteria.

    Read More

    Abcam Scientific Support

    Answered on Dec 08 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Vector MOM Kit Mouse IgG as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.6% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Mr. Manoj Kumar Valluru

    Verified customer

    Submitted May 24 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (prostate)
    Permeabilization
    Yes - 0.2% Triton-X
    Specification
    prostate
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 21 2009

    Western blot abreview for Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (prostate tumor cell line)
    Gel Running Conditions
    Reduced Denaturing (10% Tris-gly)
    Loading amount
    50 µg
    Specification
    prostate tumor cell line
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 21 2009

    Western blot abreview for Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris 4-12%)
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 01 2008

    Western blot abreview for Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (pancreatic cancer cell line)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    pancreatic cancer cell line
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 22°C
    Read More

    Dr. Hannah Whiteman

    Verified customer

    Submitted Jul 10 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (pancreatic cancer cell lines)
    Permeabilization
    Yes - 0.1% Triton X
    Specification
    pancreatic cancer cell lines
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Hannah Whiteman

    Verified customer

    Submitted Jun 18 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF-7 breast cancer cells)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    MCF-7 breast cancer cells
    Blocking step
    Fish Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 14 2008

    1-10 of 14 Abreviews or Q&A

    •  Previous
    • 1
    • 2
    • Next 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.