Key features and details
- Rabbit monoclonal [EP1629Y] to Cytokeratin 8 (phospho S23)
- Suitable for: IP, WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cytokeratin 8 (phospho S23) antibody [EP1629Y]
See all Cytokeratin 8 primary antibodies
DescriptionRabbit monoclonal [EP1629Y] to Cytokeratin 8 (phospho S23)
Specificityab76584 detects Cytokeratin 8 phosphorylated on serine 23.
Tested applicationsSuitable for: IP, WB, IHC-P, ICCmore details
Species reactivityReacts with: Human
A phospho specific peptide corresponding to residues surrounding serine 23 of human Cytokeratin 8
- HeLa cell lysates, untreated or treated with Calyculin; human gastric adenocarcinoma and human breast adenocarcinoma tissues.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 54 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
ab76584, at a 1/100 dilution, staining Cytokeratin 8 in paraffin embedded human gastric adenocarcinoma (image 1) and human breast adenocarcinoma (image 2) tissues by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584) at 1/50000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate treated with Calyculin
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
ab76584 has been referenced in 2 publications.
- Kuga T et al. FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes. Sci Rep 6:26557 (2016). PubMed: 27222304
- Kakehashi A et al. Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas. Toxicol Appl Pharmacol 238:71-9 (2009). IHC-Fr . PubMed: 19409407