The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Is unsuitable for Flow Cyt.
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in disease
Belongs to the intermediate filament family.
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Kuga T et al. A novel mechanism of keratin cytoskeleton organization through casein kinase Ia and FAM83H in colorectal cancer. J Cell Sci126:4721-31 (2013).
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