Key features and details
- Rabbit monoclonal [E431-2] to Cytokeratin 8 (phospho S73)
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cytokeratin 8 (phospho S73) antibody [E431-2]
See all Cytokeratin 8 primary antibodies
DescriptionRabbit monoclonal [E431-2] to Cytokeratin 8 (phospho S73)
SpecificityThe antibody only detects Keratin 8 phosphorylated on Serine 73. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Full length native protein (purified) corresponding to Human Cytokeratin 8. Isolated from cells pretreated with okadaic acid or pervanadate to promote Serine hyperphosphorylation,
- WB: A431 cell lysate ICC/IF: A431 cells IHC: liver carcinoma
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32579 has been referenced in 9 publications.
- Chen X et al. Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages. Biosci Rep 39:N/A (2019). PubMed: 30760630
- Baek A et al. Autophagy and KRT8/keratin 8 protect degeneration of retinal pigment epithelium under oxidative stress. Autophagy 13:248-263 (2017). PubMed: 28045574
- Corfe BM et al. Inflammation decreases keratin level in ulcerative colitis; inadequate restoration associates with increased risk of colitis-associated cancer. BMJ Open Gastroenterol 2:e000024 (2015). WB . PubMed: 26462276
- Kuga T et al. A novel mechanism of keratin cytoskeleton organization through casein kinase Ia and FAM83H in colorectal cancer. J Cell Sci 126:4721-31 (2013). WB ; Human . PubMed: 23902688
- Arentz G et al. Increased Phospho-Keratin 8 Isoforms in Colorectal Tumors Associated with EGFR Pathway Activation and Reduced Apoptosis. ISRN Mol Biol 2012:706545 (2012). PubMed: 27398237
- Kongara S et al. Autophagy regulates keratin 8 homeostasis in mammary epithelial cells and in breast tumors. Mol Cancer Res 8:873-84 (2010). WB ; Mouse . PubMed: 20530580
- Betanzos A et al. Evidence for cross-reactivity of JAM-C antibodies: implications for cellular localization studies. Biol Cell 101:441-53 (2009). WB ; Human . PubMed: 19143587
- Kakehashi A et al. Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas. Toxicol Appl Pharmacol 238:71-9 (2009). IHC-Fr ; Rat . PubMed: 19409407
- Tao GZ et al. Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies. Exp Cell Res 312:411-22 (2006). IF ; Human . PubMed: 16343483