Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Cytokeratin 8+18 antibody [5D3]
See all Cytokeratin 8+18 primary antibodies -
DescriptionMouse monoclonal [5D3] to Cytokeratin 8+18
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Cytokeratins from the human breast carcinoma cell line MCF-7.
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Positive control
- Skin
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General notes
This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant -
PurityTissue culture supernatant
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Primary antibody notesThis antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
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ClonalityMonoclonal
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Clone number5D3
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab17139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 21102523 | |
| IHC-P | 1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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RelevanceFunction: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
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Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 3856 Human
- Entrez Gene: 3875 Human
- Omim: 148060 Human
- Omim: 148070 Human
- SwissProt: P05787 Human
- Unigene: 406013 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
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Alternative names
- CARD2 antibody
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)](https://www.abcam.com/ps/products/17/ab17139/Images/ab17139_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)ab17139 staining human mammacarcinoma by IHC-P. -
![Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)](https://www.abcam.com/ps/products/17/ab17139/Images/Cytokeratin-818-Primary-antibodies-ab17139-1.jpg)
Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)Overlay histogram showing MCF7 cells stained with ab17139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17139, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)](https://www.abcam.com/ps/products/17/ab17139/Images/Cytokeratin-818-Primary-antibodies-ab17139-8.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)This image is courtesy of an Abreview submitted by Sharon Sneddonab17139 staining Cytokeratin 8+18 in MCF7 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour at room temperature. The secondary antibody was Alexa Fluor 488®-conjugated Goat anti-Mouse, diluted 1/1000.
Protocols
References
This product has been referenced in:
- Wang Y et al. Transforming growth factor-ß regulates the sphere-initiating stem cell-like feature in breast cancer through miRNA-181 and ATM. Oncogene : (2010). ICC/IF ; Human . Read more (PubMed: 21102523) »
Customer reviews and Q&As
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