Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

Reviews (1)Q&A (1)References (1)

Overview

  • Product name
    Anti-Cytokeratin 8+18 antibody [5D3]
    See all Cytokeratin 8+18 primary antibodies
  • Description
    Mouse monoclonal [5D3] to Cytokeratin 8+18
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cytokeratins from the human breast carcinoma cell line MCF-7.

  • Positive control
    • Skin
  • General notes


    This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
  • Clonality
    Monoclonal
  • Clone number
    5D3
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 21102523
IHC-P 1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • CARD2 antibody
      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK18 antibody
      • CK8 antibody
      • CYK18 antibody
      • CYK8 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin 8 antibody
      • Cytokeratin-8 antibody
      • K18 antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 18 antibody
      • Keratin 8 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 18 antibody
      • keratin type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • KRT18 antibody
      • KRT8 antibody
      • type II cytoskeletal 8 antibody
      • Type-II keratin Kb8 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      ab17139 staining human mammacarcinoma by IHC-P.
    • Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

      Overlay histogram showing MCF7 cells stained with ab17139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17139, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)This image is courtesy of an Abreview submitted by Sharon Sneddon
      ab17139 staining Cytokeratin 8+18 in MCF7 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour at room temperature. The secondary antibody was Alexa Fluor 488®-conjugated Goat anti-Mouse, diluted 1/1000.

      See Abreview

    References

    This product has been referenced in:
    • Wang Y  et al. Transforming growth factor-ß regulates the sphere-initiating stem cell-like feature in breast cancer through miRNA-181 and ATM. Oncogene : (2010). ICC/IF ; Human . Read more (PubMed: 21102523) »
    See 1 Publication for this product

    Publishing research using ab17139? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Interested in one of your keratin 8 +18 antibodies. I'd like to use it on human formalin fixed parafin embedded (FFPE) liver samples that are 5 microns thick. Which clone would you recommend for this as there are several different available? Thanks,

    Read More
    Answer

    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Cytokeratin 8+18 antibody- antibody tested in the species and application you are using should be suitable for your applications.

    Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

    Taking this into consideration, my recommendations would be ab17139 or ab15224.

    https://www.abcam.com/Cytokeratin-8-18-antibody-5D3-ab17139.html

    https://www.abcam.com/Cytokeratin-8-18-antibody-SP141-ab15224.html



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8+18 antibody [5D3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF7 Breast Cancer Cells)
    Specification
    MCF7 Breast Cancer Cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Dr. Sharon Sneddon

    Verified customer

    Submitted Nov 30 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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