Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

Reviews (1)Q&A (1)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
  • Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

Key features and details

  • Mouse monoclonal [5D3] to Cytokeratin 8+18
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

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Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
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  • Success from the first experiment – confirmed specificity through extensive validation
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Overview

  • Product name

    Anti-Cytokeratin 8+18 antibody [5D3]
    See all Cytokeratin 8+18 primary antibodies

  • Description

    Mouse monoclonal [5D3] to Cytokeratin 8+18

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human Cytokeratin 8+18. Cytokeratins from the human breast carcinoma cell line MCF-7

  • Positive control

    • IHC-P: Human Skin tissue. Flow cyt: MCF7 cells.

  • General notes


    This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Primary antibody notes

    This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

  • Clonality

    Monoclonal

  • Clone number

    5D3

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration. PubMed: 21102523
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
Notes
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 21102523
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • CARD2 antibody
      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK18 antibody
      • CK8 antibody
      • CYK18 antibody
      • CYK8 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin 8 antibody
      • Cytokeratin-8 antibody
      • K18 antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 18 antibody
      • Keratin 8 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 18 antibody
      • keratin type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • KRT18 antibody
      • KRT8 antibody
      • type II cytoskeletal 8 antibody
      • Type-II keratin Kb8 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      Paraffin embedded human skin tissue stained for Cytokeratin 8/18 using ab 17139 in immunohistochemical analysis.
    • Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

      Overlay histogram showing MCF7 cells stained with ab17139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17139, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    References (5)

    Publishing research using ab17139? Please let us know so that we can cite the reference in this datasheet.

    ab17139 has been referenced in 5 publications.

    • Yang BY  et al. Porous Se@SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species-NF-?B pathway inhibition. Acta Biomater 88:392-405 (2019). PubMed: 30753941
    • Bhat V  et al. Isolation and characterization of a new basal-like luminal progenitor in human breast tissue. Stem Cell Res Ther 10:269 (2019). PubMed: 31443683
    • Ramachandra Rao S  et al. Compromised phagosome maturation underlies RPE pathology in cell culture and whole animal models of Smith-Lemli-Opitz Syndrome. Autophagy 14:1796-1817 (2018). PubMed: 29979914
    • Xu D  et al. Enabling autologous human liver regeneration with differentiated adipocyte stem cells. Cell Transplant 23:1573-84 (2014). PubMed: 24148223
    • Wang Y  et al. Transforming growth factor-ß regulates the sphere-initiating stem cell-like feature in breast cancer through miRNA-181 and ATM. Oncogene : (2010). ICC/IF ; Human . PubMed: 21102523

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8+18 antibody [5D3]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF7 Breast Cancer Cells)
    Specification
    MCF7 Breast Cancer Cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Dr. Sharon Sneddon

    Verified customer

    Submitted Nov 30 2011

    Question

    Interested in one of your keratin 8 +18 antibodies. I'd like to use it on human formalin fixed parafin embedded (FFPE) liver samples that are 5 microns thick. Which clone would you recommend for this as there are several different available? Thanks,

    Read More
    Answer

    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Cytokeratin 8+18 antibody- antibody tested in the species and application you are using should be suitable for your applications.

    Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

    Taking this into consideration, my recommendations would be ab17139 or ab15224.

    https://www.abcam.com/Cytokeratin-8-18-antibody-5D3-ab17139.html

    https://www.abcam.com/Cytokeratin-8-18-antibody-SP141-ab15224.html



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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