Overview

  • Product name

    Anti-Cytokeratin 8+18 antibody [KRT8.18/1346]
    See all Cytokeratin 8+18 primary antibodies
  • Description

    Mouse monoclonal [KRT8.18/1346] to Cytokeratin 8+18
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Cytokeratin 8+18. Cytoskeleton preparation from HeLa cells.

  • Positive control

    • Human colon and prostate carcinoma tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab219335 was purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    KRT8.18/1346
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab219335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • CARD2 antibody
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK18 antibody
    • CK8 antibody
    • CYK18 antibody
    • CYK8 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin 8 antibody
    • Cytokeratin-8 antibody
    • K18 antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 18 antibody
    • Keratin 8 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 18 antibody
    • keratin type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • KRT18 antibody
    • KRT8 antibody
    • type II cytoskeletal 8 antibody
    • Type-II keratin Kb8 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling Cytokeratin 8 + 18 with ab219335 at 1 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Cytokeratin 8 + 18 with ab219335 at 1 µg/ml.

References

ab219335 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab219335.
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