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  1. Link

    cytokeratin-818-antibody-krt8181346-bsa-and-azide-free-ab269768.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Anti-Cytokeratin 8+18 antibody [KRT8.18/1346] - BSA and Azide free (ab269768)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody (ab269768)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody (ab269768)

Key features and details

  • Mouse monoclonal [KRT8.18/1346] to Cytokeratin 8+18 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Cytokeratin 8+18 antibody [KRT8.18/1346] - BSA and Azide free
    See all Cytokeratin 8+18 primary antibodies
  • Description

    Mouse monoclonal [KRT8.18/1346] to Cytokeratin 8+18 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Cytokeratin 8+18. Cytoskeleton preparation from HeLa cells.

  • Positive control

    • Human colon and prostate carcinoma tissues.
  • General notes

    ab269768 is a carrier free version of ab219335.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab219335 was purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    KRT8.18/1346
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Alternative Versions

    • Anti-Cytokeratin 8+18 antibody [KRT8.18/1346] (ab219335)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 3856 Human
    • Entrez Gene: 3875 Human
    • Omim: 148060 Human
    • Omim: 148070 Human
    • SwissProt: P05787 Human
    • Unigene: 533782 Human
    • Unigene: 708445 Human
    • Alternative names

      • CARD2 antibody
      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK18 antibody
      • CK8 antibody
      • CYK18 antibody
      • CYK8 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin 8 antibody
      • Cytokeratin-8 antibody
      • K18 antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 18 antibody
      • Keratin 8 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 18 antibody
      • keratin type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • KRT18 antibody
      • KRT8 antibody
      • type II cytoskeletal 8 antibody
      • Type-II keratin Kb8 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody (ab269768)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody (ab269768)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling Cytokeratin 8 + 18 with ab219335 at 1 µg/ml.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219335).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody (ab269768)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody (ab269768)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Cytokeratin 8 + 18 with ab219335 at 1 µg/ml.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219335).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab269768? Please let us know so that we can cite the reference in this datasheet.

    ab269768 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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