Overview

  • Product name

    Anti-Cytokeratin 8+18 antibody [KRT8/803 + KRT18/835] - BSA and Azide free
    See all Cytokeratin 8+18 primary antibodies
  • Description

    Mouse monoclonal [KRT8/803 + KRT18/835] to Cytokeratin 8+18 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant full length protein corresponding to Human Cytokeratin 8+18 aa 1-483.
    Database link: P05787

    Recombinant full length protein corresponding to Human Cytokeratin 8+18 aa 1-430.
    Database link: P05783

  • Positive control

    • IHC-P: Human lung carcinoma, ovarian carcinoma and colon carcinoma tissues.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

Properties

Applications

Our Abpromise guarantee covers the use of ab216022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.5-1µg for 106 cells.
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 48 kDa.

Target

  • Relevance

    Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • CARD2 antibody
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK18 antibody
    • CK8 antibody
    • CYK18 antibody
    • CYK8 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin 8 antibody
    • Cytokeratin-8 antibody
    • K18 antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 18 antibody
    • Keratin 8 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 18 antibody
    • keratin type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • KRT18 antibody
    • KRT8 antibody
    • type II cytoskeletal 8 antibody
    • Type-II keratin Kb8 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Cytokeratin 8+18 with ab216022. Magnification: 20x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling Cytokeratin 8+18 with ab216022. Magnification: 20x.

References

This product has been referenced in:

  • Tan HS  et al. KRT8 upregulation promotes tumor metastasis and is predictive of a poor prognosis in clear cell renal cell carcinoma. Oncotarget 8:76189-76203 (2017). Read more (PubMed: 29100303) »
See 1 Publication for this product

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