RecombinantRabMAb

Recombinant Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
  • Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
  • Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10932-62] to Cytokeratin 9 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free
    See all Cytokeratin 9 primary antibodies

  • Description

    Rabbit monoclonal [EPR10932-62] to Cytokeratin 9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Cytokeratin 9. The exact sequence is proprietary.
    Database link: P35527

  • General notes

    Ab240165 is the carrier-free version of ab171966. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
IP
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.

    • Tissue specificity

      Expressed in the terminally differentiated epidermis of palms and soles.

    • Involvement in disease

      Defects in KRT9 are a cause of palmoplantar keratoderma epidermolytic (EPPK) [MIM:144200]; also abbreviated as EHPPK. EPPK is a dermatological disorder characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins. Some patients may present with knuckle pads, thick pads of skin appearing over the proximal phalangeal joints.

    • Sequence similarities

      Belongs to the intermediate filament family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CK 9 antibody
      • CK-9 antibody
      • Cytokeratin 9 antibody
      • Cytokeratin-9 antibody
      • Cytokeratin9 antibody
      • EPPK antibody
      • K1C9_HUMAN antibody
      • K9 antibody
      • Keratin 9 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 9 antibody
      • Keratin-9 antibody
      • Keratin9 antibody
      • KRT 9 antibody
      • Krt9 antibody
      • Spermatid Perinuclear Ring Manchette Protein K9 antibody
      • type I cytoskeletal 9 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing +ve staining in Human skin tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing -ve staining in Human normal uterus tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing -ve staining in Human thyroid gland carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing -ve staining in Human spleen tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing -ve staining in Human colon adenocarcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling Cytokeratin 9, using ab171966 at 1/10 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue, labeling Cytokeratin 9 using ab171966 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240165? Please let us know so that we can cite the reference in this datasheet.

    ab240165 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab240165.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com