Recombinant
RabMAb

Recombinant Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

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Overview

  • Product name

    Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free
    See all Cytokeratin 9 primary antibodies

  • Description

    Rabbit monoclonal [EPR10932-62] to Cytokeratin 9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Cytokeratin 9. The exact sequence is proprietary.
    Database link: P35527

  • General notes

    Ab240165 is the carrier-free version of ab171966. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10932-62

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.

    • Tissue specificity

      Expressed in the terminally differentiated epidermis of palms and soles.

    • Involvement in disease

      Defects in KRT9 are a cause of palmoplantar keratoderma epidermolytic (EPPK) [MIM:144200]; also abbreviated as EHPPK. EPPK is a dermatological disorder characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins. Some patients may present with knuckle pads, thick pads of skin appearing over the proximal phalangeal joints.

    • Sequence similarities

      Belongs to the intermediate filament family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CK 9 antibody
      • CK-9 antibody
      • Cytokeratin 9 antibody
      • Cytokeratin-9 antibody
      • Cytokeratin9 antibody
      • EPPK antibody
      • K1C9_HUMAN antibody
      • K9 antibody
      • Keratin 9 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 9 antibody
      • Keratin-9 antibody
      • Keratin9 antibody
      • KRT 9 antibody
      • Krt9 antibody
      • Spermatid Perinuclear Ring Manchette Protein K9 antibody
      • type I cytoskeletal 9 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing +ve staining in Human skin tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing -ve staining in Human normal uterus tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing -ve staining in Human thyroid gland carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing -ve staining in Human spleen tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      ab171966 showing -ve staining in Human colon adenocarcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling Cytokeratin 9, using ab171966 at 1/10 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] - BSA and Azide free (ab240165)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue, labeling Cytokeratin 9 using ab171966 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171966).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab240165 has not yet been referenced specifically in any publications.

    Publishing research using ab240165? Please let us know so that we can cite the reference in this datasheet.

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