Overview

  • Product name
    Anti-Cytokeratin 9 antibody [Ks9.70/Ks9.216]
    See all Cytokeratin 9 primary antibodies
  • Description
    Mouse monoclonal [Ks9.70/Ks9.216] to Cytokeratin 9
  • Host species
    Mouse
  • Specificity
    Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/granulosum) of the epidermis of palm and sole. CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    IETYHNLLEG GQEDFESSGA GKIGLGGR

    , corresponding to amino acids 450 - 477 of Human Cytokeratin 9. Synthetic peptide: RQFSSSYLTS GGGGGGGLGS GGSIR, corresponding to amino acids 4 - 28 of Human Cytokeratin 9.

  • General notes
    Isotypes IgG1 (Ks9.70) and IgG3 (Ks9.216)


    The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
  • Clonality
    Monoclonal
  • Clone number
    Ks9.70/Ks9.216
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IHC-Fr 1/20.
ICC/IF 1/10.
IHC-P 1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval (microwave method) before commencing with IHC staining protocol; in some cases protease digestion is most suitable.

Target

  • Function
    May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
  • Tissue specificity
    Expressed in the terminally differentiated epidermis of palms and soles.
  • Involvement in disease
    Defects in KRT9 are a cause of palmoplantar keratoderma epidermolytic (EPPK) [MIM:144200]; also abbreviated as EHPPK. EPPK is a dermatological disorder characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins. Some patients may present with knuckle pads, thick pads of skin appearing over the proximal phalangeal joints.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CK 9 antibody
    • CK-9 antibody
    • Cytokeratin 9 antibody
    • Cytokeratin-9 antibody
    • Cytokeratin9 antibody
    • EPPK antibody
    • K1C9_HUMAN antibody
    • K9 antibody
    • Keratin 9 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 9 antibody
    • Keratin-9 antibody
    • Keratin9 antibody
    • KRT 9 antibody
    • Krt9 antibody
    • Spermatid Perinuclear Ring Manchette Protein K9 antibody
    • type I cytoskeletal 9 antibody
    see all

References

ab19124 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you very much for your interest our Abproducts. To our knowledge, ab/xxx/ has not been tested in mouse or rat. That customer inquiried about our testing discount program, but never signed up for it. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

Plus the second immunogen from a.a. 4 - 28 does exist. You can see the Swiss Prot entry here: http://www.uniprot.org/uniprot/P35527

Please let me know if you have any questions about this offer and I would be happy to help you further.

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Answer

Thank you for contacting us and for your patience while I have prepared these documents. We are only able to provide a Certificate of Compliance, so I have these for ab59691 and ab19124 for the lots that you have specified. Please let me know if you have any questions or if there is anything else that I can do for you.

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Answer

Thank you very much for your message which has been forwarded to me as my colleague Saun is no longer in the office. The blast results you have provided from rat, this indicates approximately 71% alignment. We recommend that alignment should be over 85% to predict that an antibody will detect in a different species. At 71%, this is quite low but there may be some crossreactivity. Therefore, I can offer a discount off a future purchase if you buy ab19124 now, test it in rat and submit feedback to us in the form of an Abreview. I have placed more details below. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab19124 in rat. I will then send a discount code. This code must be issued before purchasing ab19124 so please wait for my reply before ordering. 2. Purchase ab19124 either by phone, fax, or online (www.abcam.com). 3. Test it in rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab19124 turns out to be unsuitable for rat, you will still receive the discount on your next purchase after your Abreview has been submitted. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. With regards to purchasnig from China, I can provide you with a discount code, then when you place your order with the Chinese distributor, you can let them know you have a discount code. I would encourage you to purchase frmo one of our official distributors which can be found on the following webpage (please ensure China is seletected as a country in the top right hand corner of the page). https://www.abcam.com/index.html?pageconfig=contactus Please let me know if you have any questions about this offer and I would be happy to help you further.

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Answer

I'm sorry to hear you are experiencing problems with ab19124. I would be grateful if you could please clarify a few details of your protocol so I can better understand your problem: could you confirm if you see the cytokeratin 9 band as well as a non specific band; could you also confirm the size of the non specific band you observe. It would be most useful to be able to see an image of your blot please. I would like to already suggest to decrease the blocking to 5% milk for 1 hour and to try to incubate the primary antibody in TBST only, as well as the secondary too. I would also recommend to try the secondary antibody alone to see if it is responsible for the non specific band. I look forward to hearing from you regarding details of the bands and hope the change in blocking will already help you.

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