Anti-Cytokeratin 9 antibody [Ks9.70/Ks9.216] (ab19124)


  • Product name
    Anti-Cytokeratin 9 antibody [Ks9.70/Ks9.216]
    See all Cytokeratin 9 primary antibodies
  • Description
    Mouse monoclonal [Ks9.70/Ks9.216] to Cytokeratin 9
  • Host species
  • Specificity
    Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/granulosum) of the epidermis of palm and sole. CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 450 - 477 of Human Cytokeratin 9. Synthetic peptide: RQFSSSYLTS GGGGGGGLGS GGSIR, corresponding to amino acids 4 - 28 of Human Cytokeratin 9.

  • General notes
    Isotypes IgG1 (Ks9.70) and IgG3 (Ks9.216)

    The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
  • Clonality
  • Clone number
  • Research areas


Our Abpromise guarantee covers the use of ab19124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IHC-Fr 1/20.
ICC/IF 1/10.
IHC-P 1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval (microwave method) before commencing with IHC staining protocol; in some cases protease digestion is most suitable.


  • Function
    May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
  • Tissue specificity
    Expressed in the terminally differentiated epidermis of palms and soles.
  • Involvement in disease
    Defects in KRT9 are a cause of palmoplantar keratoderma epidermolytic (EPPK) [MIM:144200]; also abbreviated as EHPPK. EPPK is a dermatological disorder characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins. Some patients may present with knuckle pads, thick pads of skin appearing over the proximal phalangeal joints.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CK 9 antibody
    • CK-9 antibody
    • Cytokeratin 9 antibody
    • Cytokeratin-9 antibody
    • Cytokeratin9 antibody
    • EPPK antibody
    • K1C9_HUMAN antibody
    • K9 antibody
    • Keratin 9 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 9 antibody
    • Keratin-9 antibody
    • Keratin9 antibody
    • KRT 9 antibody
    • Krt9 antibody
    • Spermatid Perinuclear Ring Manchette Protein K9 antibody
    • type I cytoskeletal 9 antibody
    see all


ab19124 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you very much for your interest our Abproducts. To our knowledge, ab/xxx/ has not been tested in mouse or rat. That customer inquiried about our testing discount program, but never signed up for it. However by participating in our AbTrial program...

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Thank you for contacting us and for your patience while I have prepared these documents. We are only able to provide a Certificate of Compliance, so I have these for ab59691 and ab19124 for the lots that you have specified. Please let m...

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Thank you very much for your message which has been forwarded to me as my colleague Saun is no longer in the office. The blast results you have provided from rat, this indicates approximately 71% alignment. We recommend that alignment should be ...

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I'm sorry to hear you are experiencing problems with ab19124. I would be grateful if you could please clarify a few details of your protocol so I can better understand your problem: could you confirm if you see the cytokeratin 9 band as well as a no...

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