Key features and details
- Mouse monoclonal [Ks9.70/Ks9.216] to Cytokeratin 9
- Suitable for: WB, IHC-Fr, ICC/IF, IHC-P
- Reacts with: Human
Product nameAnti-Cytokeratin 9 antibody [Ks9.70/Ks9.216]
See all Cytokeratin 9 primary antibodies
DescriptionMouse monoclonal [Ks9.70/Ks9.216] to Cytokeratin 9
SpecificitySpecifically reactive in the middle/upper suprabasal layers (stratum spinosum/granulosum) of the epidermis of palm and sole. CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum.
Tested applicationsSuitable for: WB, IHC-Fr, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
General notesIsotypes IgG1 (Ks9.70) and IgG3 (Ks9.216)
The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Primary antibody notesThe antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
Our Abpromise guarantee covers the use of ab19124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.|
|IHC-P||1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval (microwave method) before commencing with IHC staining protocol; in some cases protease digestion is most suitable.
FunctionMay serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
Tissue specificityExpressed in the terminally differentiated epidermis of palms and soles.
Involvement in diseaseDefects in KRT9 are a cause of palmoplantar keratoderma epidermolytic (EPPK) [MIM:144200]; also abbreviated as EHPPK. EPPK is a dermatological disorder characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins. Some patients may present with knuckle pads, thick pads of skin appearing over the proximal phalangeal joints.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CK 9 antibody
- CK-9 antibody
- Cytokeratin 9 antibody
ab19124 has not yet been referenced specifically in any publications.