Anti-Cytokeratin antibody [CK-cocktail] (ab115959)
Key features and details
- Mouse monoclonal [CK-cocktail] to Cytokeratin
- Suitable for: IHC-Fr, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin antibody [CK-cocktail]
See all Cytokeratin primary antibodies -
Description
Mouse monoclonal [CK-cocktail] to Cytokeratin -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human epidermal Cytokeratin.
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Positive control
- Human skin, lung, colon, prostate, or any GI tissue or cancer tissues.
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General notes
This product was changed from ascites to tissue culture supernatant on 15th June 2017. Lot numbers higher than GR309863-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
CK-cocktail -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Target
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Function
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). -
Tissue specificity
The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis. -
Involvement in disease
Defects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsUndergoes deimination of some arginine residues (citrullination). -
Cellular localization
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells. - Information by UniProt
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Database links
- Entrez Gene: 3848 Human
- Omim: 139350 Human
- SwissProt: P04264 Human
- Unigene: 80828 Human
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Alternative names
- 67 kDa cytokeratin antibody
- CK-1 antibody
- CK1 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab115959 has been referenced in 7 publications.
- Howden SE et al. Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk. Cell Stem Cell 28:671-684.e6 (2021). PubMed: 33378647
- Combes AN et al. Single cell analysis of the developing mouse kidney provides deeper insight into marker gene expression and ligand-receptor crosstalk. Development 146:N/A (2019). PubMed: 31118232
- Held M et al. Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy. PLoS One 13:e0199918 (2018). PubMed: 30048451
- Combes AN et al. Haploinsufficiency for the Six2 gene increases nephron progenitor proliferation promoting branching and nephron number. Kidney Int 93:589-598 (2018). PubMed: 29217079
- Lefevre JG et al. Self-organisation after embryonic kidney dissociation is driven via selective adhesion of ureteric epithelial cells. Development 144:1087-1096 (2017). PubMed: 28174247
- Combes AN et al. Cap mesenchyme cell swarming during kidney development is influenced by attraction, repulsion, and adhesion to the ureteric tip. Dev Biol 418:297-306 (2016). ICC/IF . PubMed: 27346698
- Short KM et al. Global quantification of tissue dynamics in the developing mouse kidney. Dev Cell 29:188-202 (2014). PubMed: 24780737