Product nameAnti-Cytokeratin antibody [CK-cocktail]
See all Cytokeratin primary antibodies
DescriptionMouse monoclonal [CK-cocktail] to Cytokeratin
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICCmore details
Species reactivityReacts with: Human
Human epidermal Cytokeratin.
- Human skin, lung, colon, prostate, or any GI tissue or cancer tissues.
This product was changed from ascites to tissue culture supernatant on 15th June 2017. Lot numbers higher than GR309863-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab115959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||1/50 - 1/100.|
|ICC||Use at an assay dependent concentration.
Use at an assay dependent concentration
FunctionMay regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1).
Tissue specificityThe source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Involvement in diseaseDefects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsUndergoes deimination of some arginine residues (citrullination).
Cellular localizationCell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
- Information by UniProt
- 67 kDa cytokeratin antibody
- CK-1 antibody
- CK1 antibody
ab115959 has been referenced in 4 publications.
- Held M et al. Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy. PLoS One 13:e0199918 (2018). PubMed: 30048451
- Lefevre JG et al. Self-organisation after embryonic kidney dissociation is driven via selective adhesion of ureteric epithelial cells. Development 144:1087-1096 (2017). PubMed: 28174247
- Combes AN et al. Cap mesenchyme cell swarming during kidney development is influenced by attraction, repulsion, and adhesion to the ureteric tip. Dev Biol 418:297-306 (2016). ICC/IF . PubMed: 27346698
- Short KM et al. Global quantification of tissue dynamics in the developing mouse kidney. Dev Cell 29:188-202 (2014). PubMed: 24780737