Cytokine Array – Rat Cytokine Antibody Array (Membrane, 19 Targets) (ab133991)
Overview
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Product name
Cytokine Array – Rat Cytokine Antibody Array (Membrane, 19 Targets)
See all Cytokine Antibody Array antibody arrays -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Rat -
Product overview
ab133991 is for simultaneous detection of 19 Rat Cytokines in 4 or 8 samples. Suitable for all sample types.
Targets: CINC-2, CINC-3, CNTF, Fractalkine, GM-CSF, IFN-gamma, IL-1alpha, IL-1beta, IL-4, IL-6, IL-10, Leptin, LIX, MCP-1, MIP-3alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF
Learn more about membrane antibody arrays
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Notes
If you are interested in this cytokine array, a list of rat cytokine membrane arrays is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials Cytokine Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection |
Use at an assay dependent concentration.
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| Notes |
|---|
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Multiplex Protein Detection
Use at an assay dependent concentration. |
Images
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Typical results with Cytokine Arrays
Typical results obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133991 has been referenced in 1 publication.
- Leppik L et al. Role of Adult Tissue-Derived Pluripotent Stem Cells in Bone Regeneration. Stem Cell Rev Rep 16:198-211 (2020). PubMed: 31828580