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    cytokine-array--rat-cytokine-antibody-array-membrane-19-targets-ab133991.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Cytokine Array – Rat Cytokine Antibody Array (Membrane, 19 Targets) (ab133991)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a question References (1)

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Typical results with Cytokine Arrays

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    Overview

    • Product name

      Cytokine Array – Rat Cytokine Antibody Array (Membrane, 19 Targets)
      See all Cytokine Antibody Array antibody arrays
    • Sample type

      Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
    • Assay type

      Semi-quantitative
    • Species reactivity

      Reacts with: Rat
    • Product overview

      ab133991 is for simultaneous detection of 19 Rat Cytokines in 4 or 8 samples. Suitable for all sample types.


      Targets: CINC-2, CINC-3, CNTF, Fractalkine, GM-CSF, IFN-gamma, IL-1alpha, IL-1beta, IL-4, IL-6, IL-10, Leptin, LIX, MCP-1, MIP-3alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF


      Learn more about membrane antibody arrays


       

    • Notes

      If you are interested in this cytokine array, a list of rat cytokine membrane arrays is available here.

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Tested applications

      Suitable for: Multiplex Protein Detectionmore details

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 4 Membranes 1 x 8 Membranes
      1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl
      1X Blocking Buffer 1 x 25ml 2 x 25ml
      20X Wash Buffer I 1 x 10ml 1 x 20ml
      20X Wash Buffer II 1 x 10ml 1 x 20ml
      2X Cell Lysis Buffer 1 x 10ml 1 x 16ml
      8-Well Incubation Tray (with Lid) 1 unit 1 unit
      Biotin-Conjugated Anti-Cytokines 2 vials 4 vials
      Cytokine Antibody Array Membranes 4 units 8 units
      Detection Buffer C 1 x 1.5ml 1 x 2.5ml
      Detection Buffer D 1 x 1.5ml 1 x 2.5ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interferons
      • Antibody Arrays
      • Membrane antibody arrays
      • Cytokine antibody arrays

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab133991 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.
    Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.

    Images

    • Typical results with Cytokine Arrays
      Typical results with Cytokine Arrays

      Typical results obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab133991? Please let us know so that we can cite the reference in this datasheet.

    ab133991 has been referenced in 1 publication.

    • Leppik L  et al. Role of Adult Tissue-Derived Pluripotent Stem Cells in Bone Regeneration. Stem Cell Rev Rep 16:198-211 (2020). PubMed: 31828580

    Customer reviews and Q&As

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