Cytokine Array – Human Cytokine Antibody Array (Membrane, 42 Targets) (ab133997)

Overview

  • Product name
    Cytokine Array – Human Cytokine Antibody Array (Membrane, 42 Targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab133997 is a cytokine array to be used for the simultaneous detection of 42 Human cytokines. It is suitable for all sample types.


    Targets: ENA-78, GCSF, GM-CSF, GRO, GRO-alpha, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1, MCP-2, MCP-3, MCSF, MDC, MIG, MIP-1delta, RANTES, SCF, SDF-1, TARC, TGF-beta1, TNF-alpha, TNF-beta, EGF, IGF-I, Angiogenin, Oncostatin M, Thrombopoietin, VEGF-A, PDGF BB, Leptin


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about cytokine arrays and other membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, cytokine array ab133998ab133996ab169817, ab134003ab134000, ab169804 and ab169805 may also be of interest.


    A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab133997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.

    Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control.

  • The antibody array was used to determine the change in expression of cytokines related to cell proliferation.

     

    One million Human breast cancer cells (MCF7) were cultured in a 10mm dish for 48 hours (in 8ml of RPMI-1640 medium) and were then treated with 5 mM Compound X (not disclosed) for 48 hours. The Abcam protocol was followed for the rest of the procedure. 1 mL of supernatant of culture medium was used for sample incubation (4°C overnight).

     

    Rating: 4.5/5

  • Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

  • Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997.

  • Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997. Mean pixel density was quantified using CCD camera software analysis.

Protocols

References

This product has been referenced in:
  • Roumane A  et al. Caspase-independent cell death does not elicit a proliferative response in melanoma cancer cells. BMC Cell Biol 19:11 (2018). Read more (PubMed: 29973136) »
  • Pihlaja R  et al. The Anti-Inflammatory Effects of Lipoxygenase and Cyclo-Oxygenase Inhibitors in Inflammation-Induced Human Fetal Glia Cells and the Aß Degradation Capacity of Human Fetal Astrocytes in an Ex vivo Assay. Front Neurosci 11:299 (2017). Human . Read more (PubMed: 28611577) »
See all 5 Publications for this product

Customer reviews and Q&As

1-5 of 5 Q&A

Answer

The antibody pair designated "GRO" in the array is reactive with alpha, beta, and gamma GRO. The pair for GRO alpha detects that protein exclusively and will not detect beta or gamma. Please let me know if you have any questions.

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Question
Answer

Thanks for your further questions.
Below is a list of the sensitivities of all of the targets. Please note there may be minor variations by lot:

Human Sensitivity - pg/ml
ACE-2 10000
ActinvinA 1000
Adiponectin/Acrp30 100000
Adipsin/Factor 100
AFP 100
AgRP(ART) 10000
ALCAM 100
Angiogenin 10
Angiopoietin-1 10000
Angiopoietin-2 10000
Angiostatin 10000
ANGPTL4 10000
AR (amphiregulin) 100
Axl 10000
B7-1(CD80) 100
BCAM 100
BDNF 100
Beta IG-H3 1000
bFGF 10000
BLC 10
BMP-4 100000
BMP-5 2000
BMP-6 10000
BMP-7 1
b-NGF 10000
BTC 1
CA IX 100
CA15-3 1000
Cardiotrophin-1 1
CathepsinS 10
CCL-14a 1
CCL21/6ckine 1
CCL28/VIC 100000
CD137 100
CD14 1000
CD23 1000
CD30 1000
CD40 1000
CD40 Ligand 1000
CEACAM-1 1000
Ck beta 8-1 1,000
CNTF 10000
CNTF 320
Cripto 1000
CRP 100
CTACK/CCL27 100
CTLA-4 10000
CXCL14/BRAK 100000
CXCL16 1000
DAN 100
Decorin 10
DKK-1 1
DKK-3 10
Dkk-4 100
DPPIV 100
DR6 10000
Dtk 10000
E-Cadherin 100
EGF 1
EGF R 10000
EG-VEGF 1
ENA-78 1
Endostatin 100
Eotaxin 1
Eotaxin-2 1
Eotaxin-3 320
EPO R 100
ErBb2 100
ErbB3 100000
E-Selectin 1000
Fas Ligand 2
Fas/TNFRSF6 100000
Fcr RIIB/C 1000
Ferrtin 100
FGF-4 1,000
FGF-6 1,000
FGF-7 1
FGF-9 100
FLRG 1000
Flt-3 Ligand 1
Follistatin 10000
Fractalkine 1,600
FSH 100
Furin 2000
Galectin-7 1000
GCP-2 100
GCSF 2,000
GDF-15 1
GDNF 100
GITR Ligand/TNFSF18 100000
GITR/TNFRF18 1000
GM-CSF 100
GRO alpha-1000, beta-1000, gamma-1
GRO-a 1,000
Growth Factor 1
HB-EGF 20
HCC-1 100
HCC-4/CCL16 1000
HGF 200
HVEM 100
I-309 1,000
ICAM-1 100000
ICAM-2 10000
ICAM-3 1000
IFN-gamma 100
IGFBP-1 1
IGFBP-2 10
IGFBP-3 1,000
IGFBP-4 1,000
IGFBP-5 200
IGFBP-6 1000
IGF-I 10
IGF-I SR 10000
IGF-II 10
IL-1 alpha 1,000
IL-1 beta 100
IL-1 R4/ST2 1000
IL-1 sRI 1000
IL-1 sRII 10000
IL-10 10
IL-10 R alpha 1000
IL-10 R beta 1000
IL-10(RB 100
IL-11 100000
IL-12 1
IL-12 p40 1000
IL-12 p70 100
IL-13 100
IL-13 Ra1 100
IL-13R2 100
IL-15 100
IL-16 1
IL-17 1000
IL-17B 100
IL-17C 100000
IL-17F 100
IL-17R 10
IL-18 BPa 1000
IL-18 R beta 10
IL-18 1000
IL-1bate(RB) 100
IL-1ra 10
IL-2 25
IL-2 R alpha 10
IL-2 R beta 10000
IL-2 R gamma 100
IL-2 sRa 1000
IL-21 R 100
IL-22 10
IL-28A/IFN-lambda 100
IL-29 1000
IL-3 100
IL-31 10000
IL-4 1
IL-5 1
IL-5 R alpha 1000
IL-6 1
IL-6 sR 10
IL-7 100
IL-8 1
IL-8(RB) 1
IL-9 100
IL-9 R 1
Insulin 100
IP-10 10
I-TAC/CXCL11 10000
LAP(TGF-b1) 1
Leptin R 20
LEPTIN(OB) 100
LH 100
LIF 1,000
LIGHT 1
LIMPII 10000
Lipocalin-2 1
L-Selectin 1
Lymphotactin 1,000
LYVE-1 100
Marapsin 10000
MCP-1 3
MCP-2 100
MCP-3 1,000
MCP-4 100
M-CSF 1
M-CSF R 1000
MDC 1,000
MICA 100
MICB 1000
MIF 100
MIG 1
MIP-1 beta 10
MIP-1 delta 100
MIP-1a 10
MIP-1-a 20
MIP-3 alpha 100
MIP-3b 1000
MMP-1 1000
MMP-10 100
MMP-13 100
MMP-2 10
MMP-3 10
MMP-8 1
MMP-9 1000
MPIF-1 1000
MSP a 10000
NAP-2 100
NCAM-1 10000
NGF R 100
Nidogen-1 1000
NrCAM 1000
NRG1-b1 100
NSE 10
NT-3 20
NT-4 2
ONCOSTATIN M 100
Osteoprotegerin 100
PAI-I 100
PARC 1,000
P-Cadherin 1
PDGF R alpha 10
PDGF R beta 10
PDGF-AA 100
PDGF-AB 1000
PDGF-BB 1000
PECAM-1 10000
PF4 100
PlGF 100
Procalcitonin 100
Prolactin 1000
PSA free 10
P-selectin 100
RAGE 10000
RANK 100
RANK L 10000
RANTES 2,000
Resistin 100
S-100b 100
SCF 10
SCF R 100
SDF-1 2,000
SDF-1b 100
sgp130 100
Shh N 1000
Siglec-9 10
ST2 100
sTNF RII/TNFRS1B 10
sTNT RI/TNFRS1A 100
TACE 10000
TARC 100
TECK/CCL25 10000
TGF-a 10
TGF-beta 2 1,000
TGF-beta 3 100
TGF-beta1 200
Tie-1 1000
Tie-2 10000
TIM-1 10000
TIMP-1 100
TIMP-2 1
TIMP-4 100
TNF-alpha 10
TNF-beta 1,000
TPO 100
TRAIL R1 100
TRAIL R2 100
TRAIL s R3/TNFRS10C 100
TRAIL s R4/TNFRS10D 1000
Trappin-2 1000
TREM-1 100
TSH 100
TSLP 1
u PAR 100
VCAM-1 10000
VE-Cadherin 10000
VEGF 100
VEGF R2 10000
VEGF R3 1000
VEGF-C 10000
VEGF-D 10000
XEDAR 100
Please let us know if you have further questions.

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Question
Answer

Thank you for your enquiry.

This product can detect IL-2 at concentrations of 25 to 250,000 pg/ml (a range of 10,000-fold) and has a sensitiviy of 4 pg/ml for MCP-1.

Please let me know if you have further questions.

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Answer

Thank you for your reply. I am glad you found the information provided of help.

Each membrane of the kit should be used with a total volume of 1 mL of sample. Depending what kind of sample you are using, this may need to be pre-diluted to obtain the optimum results. A guideline of recommended sample dilutions can be found from the kit protocol descriptions:

ab133996

https://www.abcam.com/ps/products/133/ab133996/documents/ab133996%20Cytokine%20Human%20Membrane%20Antibody%20Array%2023%20Targets%20-%20protocol%20c%20array%20v2(website).pdf

ab133997

https://www.abcam.com/ps/products/133/ab133997/documents/ab133997%20Cytokine%20Human%20Membrane%20Antibody%20Array%2042%20Targets%20-%20protocol%20c%20array%20v2(website).pdf

ab133998

https://www.abcam.com/ps/products/133/ab133998/documents/ab133998%20Cytokine%20Human%20Membrane%20Antibody%20Array%2080%20Targets%20-%20protocol%20c%20array%20v2(website).pdf

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Answer

Thank you for contacting us and your interest in our products. My colleague xxxxx passed on your enquiry to the Scientific Support team at Abcam and I will hopefully be able to help answer your question.

The antibody arrays are a unique tool, allowing for the semi-quantitative profiling of protein expression. Each one of our kits typically allows for the simultaneous profiling of 20-80 proteins in your samples. Each kit can either be purchased with 4 or 8 membranes. From this we would typically recommend performing one positive control and one negative, allowing for 2 or 6 samples to be analysed (from the 4 or 8 membrane kits respectively). More information on these kits can be found from the following link:

www.abcam.com/arrays

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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