Cytokine Array – Human Cytokine Antibody Array (Membrane, 80 Targets) (ab133998)

Overview

  • Product name
    Cytokine Array – Human Cytokine Antibody Array (Membrane, 80 Targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab133998 is for simultaneous detection of 80 Human Cytokines. Suitable for all sample types.


    Targets: ENA-78, GCSF, GM-CSF, GRO, GRO-alpha, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1, MCP-2, MCP-3, MCSF, MDC, MIG, MIP-1beta, MIP-1delta, RANTES, SCF, SDF-1, TARC, TGF-beta1, TNF-alpha, TNF-beta, EGF, IGF-I, Angiogenin, Oncostatin M, Thrombopoietin, VEGF-A, PDGF-BB, Leptin, BDNF, BLC, Ckß8-1, Eotaxin, Eotaxin-2, Eotaxin-3, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-16, IP-10, LIF, LIGHT, MCP-4, MIF, MIP-3 alpha, NAP-2, NT-3, NT-4, Osteopontin, Osteoprotegerin, PARC, PLGF, TGF-beta2, TGF-beta3, TIMP-1, TIMP-2


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, cytokine array ab133997, ab169817, ab133996, ab169811, ab169817, ab169812ab134000, ab169803, ab134002ab169804ab169805ab134003 and ab169819 may also be of interest.


    A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1 vial
    Cytokine Antibody Array Membranes 4 units 8 units 2 units
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.

    Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133998.

  • Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133998. Mean pixel density was quantified using CCD camera software analysis.

Protocols

References

This product has been referenced in:
  • Redondo-Castro E  et al. Changes in the secretome of tri-dimensional spheroid-cultured human mesenchymal stem cells in vitro by interleukin-1 priming. Stem Cell Res Ther 9:11 (2018). Read more (PubMed: 29343288) »
  • Flaherty RA  et al. Neutralization of Streptolysin S-Dependent and Independent Inflammatory Cytokine IL-1ß Activity Reduces Pathology During Early Group A Streptococcal Skin Infection. Front Cell Infect Microbiol 8:211 (2018). Read more (PubMed: 30018884) »
See all 5 Publications for this product

Customer reviews and Q&As

1-5 of 5 Q&A

Answer



It would not be recommended to use Trizol for extraction as it contains Guanidinium thiocyanate which is a strong protein denaturant. Some of our ELISA antibodies recognize conformational epitopes of the target protein so it is important that the proteins are not denatured and retain their native 3-D shape.

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Question
Answer

As indicated in the product name, you can detect 80 cytokine targets on one membrane. Please see the protocol booklet for the order of the targets.

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Answer

Thank you for your reply. I am glad you found the information provided of help.

Each membrane of the kit should be used with a total volume of 1 mL of sample. Depending what kind of sample you are using, this may need to be pre-diluted to obtain the optimum results. A guideline of recommended sample dilutions can be found from the kit protocol descriptions:

ab133996

https://www.abcam.com/ps/products/133/ab133996/documents/ab133996%20Cytokine%20Human%20Membrane%20Antibody%20Array%2023%20Targets%20-%20protocol%20c%20array%20v2(website).pdf

ab133997

https://www.abcam.com/ps/products/133/ab133997/documents/ab133997%20Cytokine%20Human%20Membrane%20Antibody%20Array%2042%20Targets%20-%20protocol%20c%20array%20v2(website).pdf

ab133998

https://www.abcam.com/ps/products/133/ab133998/documents/ab133998%20Cytokine%20Human%20Membrane%20Antibody%20Array%2080%20Targets%20-%20protocol%20c%20array%20v2(website).pdf

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Answer

Thank you for contacting us and your interest in our products. My colleague xxxxx passed on your enquiry to the Scientific Support team at Abcam and I will hopefully be able to help answer your question.

The antibody arrays are a unique tool, allowing for the semi-quantitative profiling of protein expression. Each one of our kits typically allows for the simultaneous profiling of 20-80 proteins in your samples. Each kit can either be purchased with 4 or 8 membranes. From this we would typically recommend performing one positive control and one negative, allowing for 2 or 6 samples to be analysed (from the 4 or 8 membrane kits respectively). More information on these kits can be found from the following link:

www.abcam.com/arrays

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Answer

Thank you for contacting us. Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

The smallest size that the kit ab133998 is available in is 4 membranes so the proper protocol can be followed: (1 membrane: positive control, 1 membrane: negative control, 2 membranes for samples)

Since you mentioned your human samples have low protein volume and suspected low abundance of cytokines, I've attached our sensitivity table indicating the sensitivity for each target.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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