Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 62 Targets) (ab133995)

Overview

  • Product name
    Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 62 Targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab133995 is for simultaneous detection of 62 Mouse Cytokines. Suitable for all sample types.


    Targets: Axl, BLC, CD30 L, CD30, CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1beta, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, IL-1alpha, IL-2, IL-3, IL-3 Rbeta, IL-4, IL-5, IL-6, IL-9, KC/CXCL1, Leptin/OB, Leptin R, LIX, L-Selectin, Lymphotactin, MCP1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1alpha, sTNF RI, sTNF RII, TARC, TCA-3, TECK, TIMP-1, TNFalpha, Thrombopoietin, VCAM-1, VEGF-A


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Biotinylated Antibody Cocktail (C3) 2 vials 4 vials 1 vial
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Mouse Cytokine Antibody Arrays Membranes (C3) 4 vials 8 x 1l 1 vial
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical results obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Protocols

References

This product has been referenced in:
  • Nam Y  et al. Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis. PLoS One 13:e0198740 (2018). Read more (PubMed: 29879214) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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