Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 22 Targets) (ab133993)

Overview

  • Product name
    Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 22 Targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab133993 is for simultaneous detection of 22 Mouse Cytokines. Suitable for all sample types.


    Targets: GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12p70, IL-13, IL-17, IFN-gamma, MCP-1, MCP-5, RANTES, SCF, sTNF RI, TNF-alpha, Thrombopoietin, VEGF


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, a table of mouse cytokine membrane arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1 vial
    Cytokine Antibody Array Membranes 4 units 2 x 4 units 2 units
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • The mouse cytokine antibody array containing 22 target proteins was used to determine the effect of an anti-inflammatory synthetic peptide (AISP) on the induction of cytokine production in mouse macrophages Raw264.7. Three million macrophages were plated in two sets in a six well plate and used in the study; one set was treated with vehicle alone while the other set was treated with the AISP at 20 µg/mL for 5 h. After 5 h of AISP treatment, the conditioned medium were collected and centrifuged at 14,000 rpm for 10 min and the supernatant was collected in a pre-cooled tube and stored overnight at 4 °C. The experiment was performed following the manufacturer’s instructions. All incubations were performed at 4°C for 12 h. After incubation and washing steps, the proteins were detected using the provided detection reagents. Membranes were exposed for 15 s. The representative membranes for non-AISP and the AISP treated macrophages are shown.

     

    Abreview rating 4/5 stars. Review from Abcam user community. Verified customer - Yi Chen, Ph.D. Research Fellow, Spiegelman Lab, Dana-Farber Cancer Institute, Department of Cancer Biology, Boston, USA. Submitted 25-Jun-15.

Protocols

References

This product has been referenced in:
  • Martínez-Sarrà E  et al. Human dental pulp pluripotent-like stem cells promote wound healing and muscle regeneration. Stem Cell Res Ther 8:175 (2017). Mouse . Read more (PubMed: 28750661) »
  • Huante-Mendoza A  et al. Peptide IDR-1002 Inhibits NF-?B Nuclear Translocation by Inhibition of I?Ba Degradation and Activates p38/ERK1/2-MSK1-Dependent CREB Phosphorylation in Macrophages Stimulated with Lipopolysaccharide. Front Immunol 7:533 (2016). Read more (PubMed: 27933067) »
See all 2 Publications for this product

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