Product nameCytokine Array – Mouse Cytokine Antibody Array (Membrane, 32 Targets)
See all Cytokine Antibody Array antibody arrays
Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
Species reactivityReacts with: Mouse
ab133994 is for simultaneous detection of 32 Mouse Cytokines. Suitable for all sample types.
Targets: 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40/p70, IL-12 p70, IL-13, IL-17, IFN-gamma, KC/CXCL1, Leptin/OB, MCP-1, MCP-5, MIP-1alpha, MIP-2, MIP-3beta, RANTES, SCF, sTNF RI, TARC, TIMP-1, TNF-alpha, Thrombopoietin, VEGF-A
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 2 vials Cytokine Antibody Array Membranes 4 units 8 units 2 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
Our Abpromise guarantee covers the use of ab133994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
The cytokine antibody array was used to detect changes in cytokine/chemokine secretion by cultured mouse eosinophils treated with recombinant protein of interest or with vehicle control. Briefly, mouse primary eosinophils were harvested and cultured in suspension, 300ng/ml recombinant protein X (our protein of interest) or vehicle alone were added to the cells for 24 hours. Then the conditioned medium were harvested by centrifugation and added directly without dilution onto the cytokine antibody array (the array was first blocked according to manufacturer’s protocol). Manufacturer’s protocol was followed for the subsequent steps and the end result is shown in the following picture. The amount of three candidate cytokine/chemokines were identified to have changed in the treated conditioned medium: GCSF (H1 and H2), Leptin (K3 and K4), and KC (J3 and J4). The changes of Leptin and KC secretion were subsequently confirmed by ELISA in independent experiments.
Abreview rating 4/5 stars. Review from Abcam user community. Verified customers - Yi Chen, Ph.D. Research Fellow, Spiegelman Lab, Dana-Farber Cancer Institute, Department of Cancer Biology, Boston, USA. Submitted 25-Jun-15.
ab133994 has not yet been referenced specifically in any publications.