Product nameCytokine Array – Mouse Cytokine Antibody Array (Membrane, 96 Targets)
See all Cytokine Antibody Array antibody arrays
Sample typeSerum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Species reactivityReacts with: Mouse
ab193659 can be used for the simultaneous detection of 96 Mouse Cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 2 separate membranes labelled C3 and C4 (see below for images showing the array layout on each membrane).
Target names: Axl, Bfgf, BLC (CXCL13), CD30 Ligand (TNFSF8), CD30 (TNFRSF8), CD40 (TNFRSF5), CRG-2, CTACK (CCL27), CXCL16, CD26 (DPPIV), Dtk, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), E-Selectin, Fas Ligand (TNFSF6), Fc gamma RIIB (CD32b), Flt-3 Ligand, Fractalkine (CX3CL1), GCSF, GITR (TNFRSF18), GM-CSF, HGFR, ICAM-1 (CD54) IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGF-1, IGF-2, IL-1 beta (IL-1 F2), IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17A, IL-17 RB, IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-7, IL-9, I-TAC (CXCL11), KC (CXCL1), Leptin, Leptin R, LIX, L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, MIP-2, MIP-3 beta (CCL19), MIP-3 alpha (CCL20), MMP-2, MMP-3, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Platelet Factor 4 (CXCL4), Pro-MMP-9, P-Selectin, RANTES (CCL5), Resistin, SCF, SDF-1 alpha (CXCL12 alpha), Sonic Hedgehog N-Terminal (Shh-N), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TARC (CCL17), I-309 (TCA-3/CCL1), TECK (CCL25), TCK-1 (CXCL7), TIMP-1, TIMP-2, TNF alpha, Thrombopoietin (TPO), TRANCE (TNFSF11), TROY (TNFRSF19), TSLP, VCAM-1 (CD106), VEGF-A, VEGFR1, VEGFR2, VEGFR3, VEGF-D
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 2 x 50µl 1 x 50µl 1X Blocking Buffer 2 x 25ml 4 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 20ml 2 x 20ml 1 x 10ml 20X Wash Buffer II 1 x 20ml 2 x 20ml 1 x 10ml 2X Cell Lysis Buffer 1 x 16ml 2 x 16ml 1 x 10ml 8-Well Incubation Tray (with Lid) 1 unit 2 units 1 unit Biotinylated Antibody Cocktail (C3) 2 vials 4 vials 1 vial Biotinylated Antibody Cocktail (C4) 2 vials 4 vials 1 vial Detection Buffer C 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml Mouse Cytokine Antibody Arrays Membranes (C3) 1 x 4 units 1 x 8 units 1 x 2 units Mouse Cytokine Antibody Arrays Membranes (C4) 1 x 4 units 1 x 8 units 1 x 2 units
Our Abpromise guarantee covers the use of ab193659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab193659 has been referenced in 1 publication.
- Kadam P et al. Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice. Stem Cell Res Ther 9:317 (2018). PubMed: 30463610