Cytokine Array – Rat Cytokine Antibody Array (Membrane, 34 Targets) (ab133992)

Overview

  • Product name
    Cytokine Array – Rat Cytokine Antibody Array (Membrane, 34 Targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Rat
  • Product overview

    ab133992 is for simultaneous detection of 34 Rat Cytokines. Suitable for all sample types.


    Targets: Activin A, Agrin, Thymus Chemokine-1, B7-2/CD86, CINC-1, CINC-2alpha, CINC-3, CNTF, Fas Ligand, Fractalkine, GM-CSF, ICAM-1, IFN-gamma, IL-1 R6, IL-10, IL-13, IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, Leptin, LIX, L-Selectin, MCP-1, MIP-3alpha, MMP-8, beta-NGF, PDGF-AA, Prolactin R, RAGE, TIMP-1, TNF-alpha, VEGF.


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, a table listing all of our rat membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab133992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical results obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Protocols

References

This product has been referenced in:
  • Fathi E  et al. Cytokines secreted from bone marrow derived mesenchymal stem cells promote apoptosis and change cell cycle distribution of K562 cell line as clinical agent in cell transplantation. PLoS One 14:e0215678 (2019). Read more (PubMed: 31009502) »
  • Mazzio EA  et al. Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2- in LPS/IFN? activated glioma cells. J Neuroimmunol 302:10-19 (2017). Read more (PubMed: 27956075) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer


Am I assuming correctly that you are talking about Step 5, starting on page 21 and continue on page 22?

According to the protocol I downloaded from the virtual datasheet, it should read:

5. Gently pipette 500 μl of the Detection Buffers mixture onto
each membrane (e.g., 500 μl = 250 μl of Detection Buffer C
and 250 μl of Detection Buffer D) and incubate for 2 minutes
at RT (do not rock or shake). Immediately afterwards,
proceed to Step 6.


I have attached the pdf file you can download from our website to this email.

To avoid anymore confusion, may I ask where you got this information from? Was it into the little protocol book we send out with the kit? Please let me know if this is the case, so we can update it immediately.


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