Overview

  • Product name

    Anti-Cytomegalovirus AD169 antibody (FITC)
    See all Cytomegalovirus AD169 primary antibodies
  • Description

    Goat polyclonal to Cytomegalovirus AD169 (FITC)
  • Host species

    Goat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    ab30952 recognises Cytomegalovirus strain AD169.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Cytomegalovirus AD169

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purification notes

    Chromatographically purified IgG fraction.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IHC-Fr
IHC-P
  • Application notes
    ELISA: 1/1000.
    IHC-Fr: 1/200. After antigen retrieval.
    IHC-P: 1/200. After antigen retrieval.
    WB: 1/200.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Cytomegalovirus (CMV) is a member of the Herpes virus family. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid which is embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope that is decorated with virus-specific glycoprotein spikes. The viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. Cytomegalovirus strain AD169 is a laboratory-adapted strain and appears to lack a 15kb region of the 200kb genome that is present in clinical isolates. This region contains 19 open reading frames whose functions have yet to be elucidated. AD169 is also unique in that it is unable to enter latency and nearly always assumes lytic growth upon infection.
    • Alternative names

      • HCMV antibody
      • HHV5 antibody
      • Human Herpesvirus 5 antibody
      • UL55 (strain AD169) antibody

    References

    ab30952 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you very much for your call earlier this week and for your patience while I have been in touch with the lab regarding your enquiry.



    I've heard back from the lab, and the immunogen for thisantibody is purified virions of strain AD169. Theantibody recognizes predominantly surface proteins, in particular glycoprotein B (gB), so I think this antibody will not be suitable for detecting PP65.



    If I remember correctly from our phone conversation, our PP65 antibodies were not suitable for your experimental design, but if you do have any questions about any of these antibodies or need anything else, please let me know and I'll be happy to help.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up